{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:12:52Z","timestamp":1788775972888,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T00:00:00Z","timestamp":1207180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ecdysterone has been found in a great many plants and animals and has some valuable pharmaceutical properties. The present study was conducted to investigate optimal conditions for the extraction of the compound by supercritical fluid extraction from the roots of <jats:italic>Achyranthes bidentata<\/jats:italic> BL. An orthogonal array design (OAD), OA<jats:sub>9<\/jats:sub>(3<jats:sup>4<\/jats:sup>), was employed as a chemometric method for optimization of the extraction of ecdysterone from the herbal medicine. Four parameters, namely, pressure and temperature of the supercritical fluid, the dynamic extraction time, and the flow rate of dimethyl sulfoxide, were studied and optimized by a three\u2010level OAD. Determinations of the extracts were performed by high\u2010performance liquid chromatography. The effects of the parameters were studied using analysis of variance. The results shown that the yield of ecdysterone could be influenced by the four parameters to a similar degree. The yield for DMSO\u2010modified supercritical CO<jats:sub>2<\/jats:sub> was in the range from 0.65 to 1.03 mg\/g under the selected conditions. In comparison with methanol\u2010modified supercritical CO<jats:sub>2 <\/jats:sub>and Soxhlet extraction, a higher yield was obtained when DMSO\u2010modified supercritical CO<jats:sub>2<\/jats:sub> was used.<\/jats:p>","DOI":"10.1002\/jssc.200700468","type":"journal-article","created":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T11:48:18Z","timestamp":1207223298000},"page":"1393-1398","source":"Crossref","is-referenced-by-count":17,"title":["Supercritical fluid extraction of ecdysterone from the roots of <i>Achyranthes bidentata<\/i> BL."],"prefix":"10.1002","volume":"31","author":[{"given":"Yizhe","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,30]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Chinese Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China Chemical Industry Press Beijing 2005 p. 49."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6327(1997)35:1\/2<3::AID-ARCH2>3.0.CO;2-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/arch.1044"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01925578"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.06.031"},{"key":"e_1_2_1_7_2","first-page":"241","volume":"39","author":"Yang S. 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