{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:34:50Z","timestamp":1766086490304,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2008,7,29]],"date-time":"2008-07-29T00:00:00Z","timestamp":1217289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and direct analysis using column\u2010switching HPLC method was developed and validated for the quantification of active metabolites of sibutramine,<jats:italic> N<\/jats:italic>\u2010mono\u2010desmethyl metabolite (metabolite 1, M1) and <jats:italic>N<\/jats:italic>\u2010di\u2010desmethyl metabolite (metabolite 2, M2) in the serum of rats administered sibutramine HCl (5.0 mg\/kg, p.o.). Rat serum was directly injected onto the precolumn without sample prepreparation step following dilution with mobile phase A, <jats:italic>i.\u00a0e.<\/jats:italic>, methanol\u2013ACN\u201320 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (8.3:4.5:87.2 by volume). After the endogenous serum components were eluted to waste, the system was switched and the analytes were eluted to the trap column. Active metabolites M1 and M2 were then back\u2010flushed to the analytical column for separation with mobile phase B, <jats:italic>i.\u00a0e.<\/jats:italic>, methanol\u2013ACN\u201320 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (35.8:19.2:45 by volume) and detected at 223 nm. The calibration curves of active metabolites M1 and M2 were linear in the range of 0.1\u20131.0 \u03bcg\/mL and 0.15\u20131.8 \u03bcg\/mL. This method was fully validated and shown to be specific, accurate (10.4\u201310.7% error), and precise (1.97\u20138.79% CV). This simple and rapid analytical method using column\u2010switching appears to be useful for the pharmacokinetic study of active metabolites (M1 and M2) of sibutramine.<\/jats:p>","DOI":"10.1002\/jssc.200800041","type":"journal-article","created":{"date-parts":[[2008,7,29]],"date-time":"2008-07-29T08:58:11Z","timestamp":1217321891000},"page":"2820-2826","source":"Crossref","is-referenced-by-count":13,"title":["Determination of the active metabolites of sibutramine in rat serum using column\u2010switching HPLC"],"prefix":"10.1002","volume":"31","author":[{"given":"So Young","family":"Um","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu\u2010Bong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon Hwa","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Cheol","family":"Ju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Sang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye young","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki Hwan","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeon Woo","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199243060-00003"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(00)00032-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(95)00134-4"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(93)90181-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(89)90048-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002280050565"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00818-8"},{"key":"e_1_2_1_9_2","first-page":"345","volume":"7","author":"Abolfathi Z.","year":"2004","journal-title":"J. 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