{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T15:49:26Z","timestamp":1782143366817,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2008,7,29]],"date-time":"2008-07-29T00:00:00Z","timestamp":1217289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper describes the fabrication of RP\/ion\u2010exchange mixed\u2010mode monolithic materials for capillary LC. Following deactivation of the capillary surface with 3\u2010(trimethoxysilyl)propyl methacrylate (\u03b3\u2010MAPS), monoliths were formed by copolymerisation of pentaerythritol diacrylate monostearate (PEDAS), 2\u2010sulphoethyl methacrylate (SEMA) with\/without ethylene glycol dimethacrylate (EDMA) within 100 \u03bcm id capillaries. In order to investigate the porous properties of the monoliths prepared in our laboratory, mercury intrusion porosimetry, SEM and micro\u2010HPLC were used to measure the monolithic structures. The monolithic columns prepared without EDMA showed bad mechanical stability at high pressure, which is undesirable for micro\u2010HPLC applications. However, it was observed that the small amount (5% w\/w) of EDMA clearly improved the mechanical stability of the monoliths. In order to evaluate their application for micro\u2010HPLC, a range of neutral, acidic and basic compounds was separated with these capillaries and satisfactory separations were obtained. In order to further investigate the separation mechanism of these monolithic columns, comparative studies were carried out on the poly(PEDAS\u2010<jats:italic>co<\/jats:italic>\u2010SEMA) monolithic column and two other monoliths, poly(PEDAS) and poly(PEDAS\u2010<jats:italic>co<\/jats:italic>\u20102\u2010(methacryloyloxy)ethyl\u2010trimethylammonium methylsulphate (METAM)). As expected, different selectivities were observed for the separation of basic compounds on all three monolithic columns using the same separation conditions. The mobile phase pH also showed clear influence on the retention time of basic compounds. This could be explained by ion\u2010exchange interaction between positively charged analytes and the negatively charged sulphate group.<\/jats:p>","DOI":"10.1002\/jssc.200800124","type":"journal-article","created":{"date-parts":[[2008,7,29]],"date-time":"2008-07-29T08:58:43Z","timestamp":1217321923000},"page":"2774-2783","source":"Crossref","is-referenced-by-count":21,"title":["Mixed\u2010mode reversed\u2010phase and ion\u2010exchange monolithic columns for micro\u2010HPLC"],"prefix":"10.1002","volume":"31","author":[{"given":"Zhengjin","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman W.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.06.115"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac951247v"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac061871f"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960281m"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00031a022"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.03.051"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)91309-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401772"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401721"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401825"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390136"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.090"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(04)01090-8"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048748d"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200102)22:3<544::AID-ELPS544>3.0.CO;2-8"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048747l"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401828"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600146"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.04.076"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200205)23:9<1239::AID-ELPS1239>3.0.CO;2-X"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200209)23:17<2938::AID-ELPS2938>3.0.CO;2-8"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01031-8"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01032-X"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0496695"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.10.078"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbbm.2006.08.009"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500073"},{"key":"e_1_2_1_29_2","unstructured":"Lide D. 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