{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:26:56Z","timestamp":1789741616486,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,28]],"date-time":"2009-01-28T00:00:00Z","timestamp":1233100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid chemical profiling of the antitumour active crude dichloromethane extract of the marine sponge, <jats:italic>Dactylospongia<\/jats:italic> sp. was undertaken. A combination of both offline (HPLC followed by NMR and MS) and on\u2010line (on\u2010flow and stop\u2010flow HPLC\u2010NMR) chemical profiling approaches was adopted to establish the exact nature of the major constituents present in the dichloromethane extract of this sponge. On\u2010flow HPLC\u2010NMR analysis was employed to initially identify components present in the dichloromethane extract, while stop\u2010flow HPLC\u2010NMR experiments were then conducted on the major component present, resulting in the partial identification of pentaprenylated<jats:italic> p<\/jats:italic>\u2010quinol (<jats:bold>5<\/jats:bold>). Subsequent off\u2010line RP semi\u2010preparative HPLC isolation of <jats:bold>5<\/jats:bold> followed by detailed spectroscopic analysis using NMR and MS permitted the complete structure to be established. This included the first complete carbon NMR chemical shift assignment of <jats:bold>5<\/jats:bold> based on the heteronuclear 2\u2010D NMR experiments, together with the first report of its antitumour activity. This study represents one of the few reports describing the application of HPLC\u2010NMR to chemically profile secondary metabolites from a marine organism.<\/jats:p>","DOI":"10.1002\/jssc.200800548","type":"journal-article","created":{"date-parts":[[2009,1,28]],"date-time":"2009-01-28T01:45:36Z","timestamp":1233107136000},"page":"542-548","source":"Crossref","is-referenced-by-count":16,"title":["Application of HPLC\u2010NMR for the Rapid Chemical Profiling of a Southern Australian Sponge, <i>Dactylospongia <\/i>sp."],"prefix":"10.1002","volume":"32","author":[{"given":"Daniel Anthony","family":"Dias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Urban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(00)01511-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.10.002"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/np0502037"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1574088054583273"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/np050504g"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pca.866"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-005-1305-y"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.7000057"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1075"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)81009-7"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)77183-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0690(200102)2001:4<775::AID-EJOC775>3.0.CO;2-Z"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol017271e"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja035579q"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol053092b"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2006.12.006"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/np800201c"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmra.1995.0759"},{"key":"e_1_2_1_20_2","unstructured":"Scheuer P. 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