{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:02:43Z","timestamp":1785294163550,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,3,27]],"date-time":"2009-03-27T00:00:00Z","timestamp":1238112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Herein a quantitative method for the determination of seven penicillins in bovine plasma and veterinary drugs has been developed. Amoxicillin (AMO), ampicillin (AMP), penicillin G (PENG), penicillin V (PENV), oxacillin (OXA), cloxacillin (CLO) and dicloxacillin (DICLO) were separated on a Perfectsil ODS\u20102 (250\u00d74 mm, 5 \u03bcm) column, using gradient elution, with a mobile phase of 0.1% v\/v TFA and ACN\u2013methanol (90:10 v\/v). PDA detection was used at 240 nm. Penicillins were isolated from bovine plasma by SPE on Lichrolut RP\u201018 cartridges with mean recoveries from 85.7 to 113.5%. Colchicine (3 ng\/\u03bcL) was used as an internal standard. The developed method was validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity. Repeatability (<jats:italic>n<\/jats:italic> = 5) and between\u2010day precision (<jats:italic>n<\/jats:italic> = 5) revealed RSD &lt; 12%. The detection limits in the bovine plasma were estimated as 18 ng for AMO and AMP, 25 for PENG, PENV and OXA, 3 ng for CLO and 12 ng for DICLO. Spiked plasma samples were stable for 1 wk, except for AMP and CLO, which were stable for 3 wk and OXA for 4 wk. AMO, PENG and PENV were stable for two freeze\u2013thaw cycles, OXA, CLO and DICLO for four, while AMP only for one.<\/jats:p>","DOI":"10.1002\/jssc.200800758","type":"journal-article","created":{"date-parts":[[2009,4,27]],"date-time":"2009-04-27T19:44:36Z","timestamp":1240861476000},"page":"1302-1311","source":"Crossref","is-referenced-by-count":20,"title":["Development and validation of an HPLC method for the determination of seven penicillin antibiotics in veterinary drugs and bovine blood plasma"],"prefix":"10.1002","volume":"32","author":[{"given":"Victoria F.","family":"Samanidou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios E.","family":"Giannakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Papadaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826070701274320"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2001.9695106"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(02)00068-3"},{"key":"e_1_2_1_5_2","first-page":"337","volume":"111","author":"Ehinger A. 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