{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:31:34Z","timestamp":1760920294108,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T00:00:00Z","timestamp":1248825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002772","name":"Cagliari University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002772","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Catholic University in Rome"},{"name":"MUR"},{"name":"Italian National Research Council"},{"name":"Swedish Science Council"},{"name":"LUA\/ALF agreement"},{"name":"\u201cINTERLINK\u201d agreement"},{"name":"Regione Sardegna"},{"DOI":"10.13039\/100007359","name":"Fondazione Banco di Sardegna","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007359","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two peptides (MW 1211.7 and 928.5 Da) were detected by RP\u2013HPLC\u2013ESI\u2013MS analysis of parotid saliva secreted upon continuous parasympathetic stimulation. The peptide with the higher mass (PSPFr\u2010A) corresponded to the N\u2010terminal dodecapeptide (Fragment 1\u201312) of rat parotid secretory protein (PSP), while the peptide with the lower mass (PSPFr\u2010B) corresponded to the 4\u201312 fragment of the same protein. During stimulation, the PSPFr\u2010A secretion increased, while the PSPFr\u2010B secretion decreased (HPLC\u2013ESI\u2013MS). In the presence of cycloheximide, PSPFr\u2010A was not demonstrated, while the PSPFr\u2010B secretion decreased. In the presence of aprotinin, the PSPFr\u2010B secretion was almost abolished, while the PSPFr\u2010A secretion increased to higher levels than those observed in the absence of the inhibitor. <jats:italic>In vitro<\/jats:italic> perfusion, with artificial solution, of stimulated rat parotid glands excluded that the fragments were derived from the circulation. Neither peptide occurred in enriched granule preparations from unstimulated glands. The results suggest that at least two pathways \u2013 granular and vesicular \u2013 are responsible for the generation of the two peptides. PSPFr\u2010A is the first cleavage product in both pathways. PRPFr\u2010B is probably generated from granular PSPFr\u2010A only and, at the end of the granule mediated pathway, by the action of an enzyme of the serine protease class.<\/jats:p>","DOI":"10.1002\/jssc.200900197","type":"journal-article","created":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T06:46:59Z","timestamp":1248850019000},"page":"2944-2952","source":"Crossref","is-referenced-by-count":7,"title":["RP\u2013HPLC\u2013ESI\u2013MS characterization of novel peptide fragments related to rat parotid secretory protein in parasympathetic induced saliva"],"prefix":"10.1002","volume":"32","author":[{"given":"J\u00f6rgen","family":"Ekstr\u00f6m","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanna","family":"Inzitari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Khosravani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Fanali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"Cabras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Fujita\u2010Yoshigaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiorshi","family":"Sugiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Messana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Castagnola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017241"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1996.sp003994"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990601)20:7<1373::AID-ELPS1373>3.0.CO;2-Z"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090010301"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400604"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700501-MCP200"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1076\/0924-3860(200010)38:4;1-O;FT243"},{"key":"e_1_2_1_9_2","first-page":"213","volume":"36","author":"Anderson L. 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