{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:42:01Z","timestamp":1760920921508,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"15-16","license":[{"start":{"date-parts":[[2009,7,13]],"date-time":"2009-07-13T00:00:00Z","timestamp":1247443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Austrian Science Foundation FWF"},{"name":"Genome Research project in Austria"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monolithic poly(1,2\u2010bis(<jats:italic>p<\/jats:italic>\u2010vinylphenyl)ethane (BVPE)) capillary columns were prepared by thermally initiated free radical polymerisation of 1,2\u2010bis(<jats:italic>p<\/jats:italic>\u2010vinylphenyl)ethane in the presence of inert diluents (porogens) and \u03b1,\u03b1\u2032\u2010azoisobutyronitrile (AIBN) as initiator. Polymerisations were accomplished in 200 \u03bcm ID fused silica capillaries at 65\u00b0C and for 60 min. Mercury intrusion porosimetry measurements of the polymeric RP support showed a broad bimodal pore\u2010size\u2010distribution of mesopores and small macropores in the range of 5\u2013400 nm and flow\u2010channels in the \u03bcm range. N<jats:sub>2<\/jats:sub>\u2010adsorption (BET) analysis resulted in a tremendous enhancement of surface area (101 m<jats:sup>2<\/jats:sup>\/g) of BVPE stationary phases compared to typical organic monoliths (\u02dc20 m<jats:sup>2<\/jats:sup>\/g), indicating the presence of a considerable amount of mesopores. Consequently, the adequate proportion of both meso\u2010 and (small) macropores allowed the rapid and high\u2010resolution separation of low\u2010molecular\u2010weight compounds as well as biomolecules on the same monolithic support. At the same time, the high fraction of flow\u2010channels provided enhanced column permeability. The chromatographic performance of poly(1,2\u2010bis(<jats:italic>p<\/jats:italic>\u2010vinylphenyl)ethane) capillary columns for the separation of biomolecules (proteins, oligonucleotides) and small molecules (alkyl benzenes, phenols, phenons) are demonstrated in this article. Additionally, pressure drop <jats:italic>versus<\/jats:italic> flow rate measurements of novel poly(1,2\u2010bis(<jats:italic>p<\/jats:italic>\u2010vinylphenyl)ethane) capillary columns confirmed high mechanical robustness, low swelling in organic solvents and high permeability. Due to the simplicity of monolith fabrication, comprehensive studies of the retention and separation behaviour of monolithic BVPE columns resulted in high run\u2010to\u2010run and batch\u2010to\u2010batch reproducibilities. All these attributes prove the excellent applicability of monolithic poly(1,2\u2010bis(<jats:italic>p<\/jats:italic>\u2010vinylphenyl)ethane) capillary columns for \u03bc\u2010HPLC towards a huge range of analytes of different chemistries and molecular sizes.<\/jats:p>","DOI":"10.1002\/jssc.200900211","type":"journal-article","created":{"date-parts":[[2009,7,13]],"date-time":"2009-07-13T09:03:47Z","timestamp":1247475827000},"page":"2510-2520","source":"Crossref","is-referenced-by-count":28,"title":["Monolithic poly(1,2\u2010bis(<i>p<\/i>\u2010vinylphenyl)ethane) capillary columns for simultaneous separation of low\u2010 and high\u2010molecular\u2010weight compounds"],"prefix":"10.1002","volume":"32","author":[{"given":"Andreas","family":"Greiderer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"S. 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