{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T15:48:37Z","timestamp":1775490517842,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,2,19]],"date-time":"2010-02-19T00:00:00Z","timestamp":1266537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"science and technology innovation foundation for young teachers of Shandong Agricultural University","award":["23489"],"award-info":[{"award-number":["23489"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of <jats:italic>Bupleurum falcatum<\/jats:italic>. An orthogonal array design L<jats:sub>9<\/jats:sub>(3)<jats:sup>4<\/jats:sup> was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30\u201340\u2009MPa), temperature (40\u201350\u00b0C), ethanol concentration (60\u2013100%) and time (2.5\u20133.5\u2009h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35\u2009MPa of pressure, 45\u00b0C of temperature, 80% of ethanol concentration and 3.0\u2009h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48\u2009mg\/g, respectively. Determinations of the saikosaponins were performed by HPLC.<\/jats:p>","DOI":"10.1002\/jssc.200900529","type":"journal-article","created":{"date-parts":[[2010,2,19]],"date-time":"2010-02-19T08:13:10Z","timestamp":1266567190000},"page":"1161-1166","source":"Crossref","is-referenced-by-count":12,"title":["Optimization of supercritical fluid extraction of saikosaponins from <i>Bupleurum falcatum<\/i> with orthogonal array design"],"prefix":"10.1002","volume":"33","author":[{"given":"Yinshi","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjie","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengbo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zude","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00510139"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-42797"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi1947.89.10_1367"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.22.1385"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(98)00217-1"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-960205"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5152"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.03.008"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X03001065"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0192-0561(88)90008-2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0192-0561(85)90044-X"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00369420"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2006.12.002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(97)00418-6"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800544"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1139\/O07-141"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2005.01037.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(95)00720-2"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(93)85130-J"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02931895"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22126"},{"key":"e_1_2_6_23_2","first-page":"53","volume":"23","author":"Wang L. H.","year":"2006","journal-title":"J. Shenyang Pharm. Univ."},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700468"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.07.032"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5866(98)00056-2"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2004.10.016"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2509(96)00379-X"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2006.06.003"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0045-6535(00)00332-5"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2005.01037.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(00)00557-9"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2008.10.007"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700386"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1996.325-27.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1005-9040(07)60049-X"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02269831"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2007.04.010"},{"key":"e_1_2_6_39_2","first-page":"38","volume":"19","author":"Liu H. P.","year":"2008","journal-title":"Trad. Chin. Drug Res. Clin. Pharm."},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1039\/p19750002043"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200900529","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200900529","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200900529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:07:19Z","timestamp":1760864839000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200900529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1002\/jssc.200900529"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200900529","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}