{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:42:00Z","timestamp":1760920920549,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2010,7,5]],"date-time":"2010-07-05T00:00:00Z","timestamp":1278288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Colombian Science Fund-COLCIENCIAS","award":["1101-405-20180"],"award-info":[{"award-number":["1101-405-20180"]}]},{"DOI":"10.13039\/501100002753","name":"UNIVERSIDAD NACIONAL DE COLOMBIA","doi-asserted-by":"crossref","award":["DIB 10763"],"award-info":[{"award-number":["DIB 10763"]}],"id":[{"id":"10.13039\/501100002753","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Instituto de Investigaciones Marinas y Costeras - INVEMAR"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The sponge <jats:italic>Discodermia dissoluta<\/jats:italic> is the source of the potent antimitotic compound (+)\u2010discodermolide. The relatively abundant and shallow populations of this sponge in Santa Marta, Colombia, allow for studies to evaluate the natural and biotechnological supply options of (+)\u2010discodermolide. In this work, an RP\u2010HPLC\u2010UV methodology for the quantification of (+)\u2010discodermolide from sponge samples was tested and validated. Our protocol for extracting this compound from the sponge included lyophilization, exhaustive methanol extraction, partitioning using water and dichloromethane, purification of the organic fraction in RP\u201018 cartridges and then finally retrieving the (+)\u2010discodermolide in the methanol\u2013water (80:20 v\/v) fraction. This fraction was injected into an HPLC system with an Xterra RP\u201018 column and a detection wavelength of 235\u2009nm. The calibration curve was linear, making it possible to calculate the LODs and quantification in these experiments. The intra\u2010day and inter\u2010day precision showed relative standard deviations lower than 5%. The accuracy, determined as the percentage recovery, was 99.4%. Nine samples of the sponge from the Bahamas, Bonaire, Cura\u00e7ao and Santa Marta had concentrations of (+)\u2010discodermolide ranging from 5.3 to 29.3\u2009\u03bcg\/g<jats:sup>\u22121<\/jats:sup> of wet sponge. This methodology is quick and simple, allowing for the quantification in sponges from natural environments, <jats:italic>in situ<\/jats:italic> cultures or dissociated cells.<\/jats:p>","DOI":"10.1002\/jssc.200900852","type":"journal-article","created":{"date-parts":[[2010,7,6]],"date-time":"2010-07-06T20:20:14Z","timestamp":1278447614000},"page":"2316-2321","source":"Crossref","is-referenced-by-count":1,"title":["Validation and evaluation of an HPLC methodology for the quantification of the potent antimitotic compound (+)\u2010discodermolide in the Caribbean marine sponge <i>Discodermia dissoluta<\/i>"],"prefix":"10.1002","volume":"33","author":[{"given":"Katherine","family":"Valderrama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Castellanos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sven","family":"Zea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00303a029"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9515127"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2307\/1312834"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2004.22.14_suppl.2025"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-1978(94)90026-4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2007.10.039"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/b705661n"},{"key":"e_1_2_6_9_2","unstructured":"Carballo J. L. Ya\u00f1ez B. Zub\u00eda E. Ortega M. J. Vega C. Mar. Biotecnol. DOI 10.1007\/s10126\u2010009\u20109235\u20109."},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2005.04.069"},{"key":"e_1_2_6_11_2","unstructured":"Ruiz C. A.2009. Aspectos ecol\u00f3gicos de la poblaci\u00f3n deDiscodermia dissoluta(Porifera: Demospongiae: Lithistida) en Santa Marta Caribe colombiano. B.Sc. thesis (Marine Biology) Univ. Jorge Tadeo Lozano Santa Marta 42 p\u00e1gs."},{"key":"e_1_2_6_12_2","unstructured":"Ruiz C. A. Zea.S.Bol. Inv. Mar. 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ICH London pp.1\u201310."},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00259a060"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200900852","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200900852","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200900852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:34:52Z","timestamp":1760880892000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200900852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,28]]},"references-count":19,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1002\/jssc.200900852"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200900852","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2010,7,28]]}}}