{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:45:12Z","timestamp":1760921112977,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,1,18]],"date-time":"2011-01-18T00:00:00Z","timestamp":1295308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Project for Basic Research","award":["2010CB126105"],"award-info":[{"award-number":["2010CB126105"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["20872021"],"award-info":[{"award-number":["20872021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Innovation Foundation for Graduate Students of Guizhou University","award":["2010026"],"award-info":[{"award-number":["2010026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The direct HPLC enantioseparation of a novel series of chiral pyridazin\u20103(2<jats:italic>H<\/jats:italic>)\u2010one derivatives with \u03b1<jats:italic>\u2010<\/jats:italic>aminophosphonate moiety was performed on two immobilized polysaccharide chiral stationary phases (Chiralpak IA, Chiralpak IC) using <jats:italic>n<\/jats:italic>\u2010hexane (<jats:italic>n<\/jats:italic>\u2010Hex)\/dichloromethane (DCM) mobile phase with 5% alcohol additive. Good baseline separation of the enantiomers was achieved using amylose <jats:italic>tris<\/jats:italic>\u2010(3,5\u2010dimethylphenylcarbamate) chiral stationary phases (Chiralpak IA) on analytical scale. The analytical method was further scaled up to semi\u2010preparative loading to obtain small amounts of both the enantiomers of pyridazin\u20103(2<jats:italic>H<\/jats:italic>)\u2010one derivative. The semi\u2010preparative resolution of all compounds was successfully achieved with <jats:italic>n<\/jats:italic>\u2010hexane\/dichloromethane\/ethanol (EtOH) as mobile phase using a semi\u2010preparative Chiralpak IA column. The first fractions were isolated with purities of &gt;99.9% (enantiomeric excess (e.e.), and the second fractions were obtained with purities of &gt;98.2% (enantiomeric excess). The assignment of the absolute configuration was established for the F1 fraction of compound a\u20102 by single\u2010crystal X\u2010ray diffraction method.<\/jats:p>","DOI":"10.1002\/jssc.201000705","type":"journal-article","created":{"date-parts":[[2011,1,18]],"date-time":"2011-01-18T03:32:27Z","timestamp":1295321547000},"page":"402-408","source":"Crossref","is-referenced-by-count":8,"title":["Analytical and semi\u2010preparative HPLC enantioseparation of novel pyridazin\u20103(2<i>H<\/i>)\u2010one derivatives with \u03b1\u2010aminophosphonate moiety using immobilized polysaccharide chiral stationary phases"],"prefix":"10.1002","volume":"34","author":[{"given":"Yuping","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoan","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pinaki S.","family":"Bhadury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deyu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.704"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14093676"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1195"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/hc.20296"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600800412"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00204912"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cjoc.200690296"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/10426509408020438"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf803215t"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/10426500903251373"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/ejoc.200800812","author":"Beck J.","year":"2009","journal-title":"Eur. 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