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A number of separation parameters were investigated and optimized, including pH, concentration of the separation buffer (\u03b5\u2010amino caproic acid), concentration of the triethylenetetramine (TETA) dynamic coating, the capillary internal diameter and the field strength used for the separation. The effects of between\u2010run flushing of the capillary and the data acquisition rate were also evaluated. Under the optimized conditions, a fast (&lt;5\u2009min), selective and reproducible separation of mAb charge variants was achieved under a very high electric field strength (1000\u2009V\/cm). This method also requires only a short conditioning of the capillary, with between\u2010run conditioning completed within 2\u2009min. The method was evaluated for specificity, sensitivity, linearity, accuracy and precision. The same separation conditions were applied to the rapid separation (2\u20135\u2009min) of charge variants of multiple monoclonal antibodies with p<jats:italic>I<\/jats:italic> in the range of 7.0\u20139.5. Compared with other existing methods for charge variants analysis, this method has several advantages including a short run time, rapid capillary conditioning and simple sample preparation.<\/jats:p>","DOI":"10.1002\/jssc.201000719","type":"journal-article","created":{"date-parts":[[2011,1,25]],"date-time":"2011-01-25T06:23:55Z","timestamp":1295936635000},"page":"548-555","source":"Crossref","is-referenced-by-count":100,"title":["Rapid analysis of charge variants of monoclonal antibodies with capillary zone electrophoresis in dynamically coated fused\u2010silica capillary"],"prefix":"10.1002","volume":"34","author":[{"given":"Yan","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen","family":"Isele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiying","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Ruesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,1,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1057\/palgrave.jcb.3050081"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920108786786358"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1837"},{"key":"e_1_2_7_5_2","first-page":"84","volume":"2","author":"Reichert J. 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