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The mobile phase was ACN\/aqueous solution of 15\u2009mM ammonium acetate adjusted with TFA to pH 3.0 (2:98 v\/v), in an isocratic mode. The drift tube temperature was set at 50\u00b0C and the nebulizing gas flow rate of air was 3.5\u2009L\/min for ELSD detection. Almost all of the reported degradation compounds of spectinomycin such as actinamine, actinospectinoic acid and biosynthesis intermediates such as dihydrospectinomycin diastereoisomers were baseline separated. MS was utilized for the identification of spectinomycin and its seven related substances. The method for the assay of spectinomycin was successfully validated with respect to accuracy, precision (RSD less than 2%), linearity (throughout the linear range 0.025\u20133\u2009mg\/mL, <jats:italic>r<\/jats:italic>=0.9993), sensitivity (LOD: 100\u2009ng on column) and robustness. The experimental results demonstrated that the simultaneous determination of chloride, spectinomycin and related substances is feasible in a single run, which suggests applicability in routine assays.<\/jats:p>","DOI":"10.1002\/jssc.201100065","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T08:44:22Z","timestamp":1309423462000},"page":"1811-1819","source":"Crossref","is-referenced-by-count":14,"title":["HPLC\u2010ELSD analysis of spectinomycin dihydrochloride and its impurities"],"prefix":"10.1002","volume":"34","author":[{"given":"Junyi","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,28]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"Spectam Oral Solution product information (Vetoquinol \u2013 Canada)2007."},{"key":"e_1_2_6_3_2","first-page":"1","volume":"41","author":"Cuerpo L.","year":"1994","journal-title":"FAO Food Nutr. 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