{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:28:01Z","timestamp":1760920081144,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T00:00:00Z","timestamp":1316131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A three\u2010phase hollow\u2010fiber liquid\u2010phase microextraction (HF\u2010LPME) method for the stereoselective determination of bufuralol metabolites 1\u2032\u2010oxobufuralol (1\u2032\u2010Oxo\u2010BF) and 1\u2032\u2010hydroxybufuralol (1\u2032\u2010OH\u2010BF) in microsomal preparations is described for the first time. The HPLC analysis was carried out using a Chiralcel OD\u2010H column with hexane\/2\u2010propanol\/methanol (97.5:2.0:0.5, v\/v\/v) plus 0.5% diethylamine as the mobile phase, and UV detection at 248 and 273\u2009nm. The HF\u2010LPME optimized conditions involved: <jats:italic>n<\/jats:italic>\u2010octanol as the organic solvent, 0.2\u2009mol\/L acetic acid as the acceptor phase, donor phase pH adjusted to 13, sample agitation at 1500\u2009rpm and extraction for 30\u2009min. By using this extraction procedure, the recovery rates were in the range of 63\u201369%. The method was linear over the concentration range of 100\u20135000\u2009ng\/mL for each enantiomer of 1\u2032\u2010Oxo\u2010BF (<jats:italic>r<\/jats:italic>&gt;0.9978) and of 100\u20132500\u2009ng\/mL for each stereoisomer of 1\u2032\u2010OH\u2010BF (<jats:italic>r<\/jats:italic>&gt;0.9957). The quantification limits were 100\u2009ng\/mL for all analytes. The validated method was used to assess the <jats:italic>in vitro<\/jats:italic> biotransformation of bufuralol using rat liver microsomal fraction that demonstrated predominant formation of (<jats:italic>S<\/jats:italic>)\u20101\u2032\u2010Oxo\u2010BF and (<jats:italic>R<\/jats:italic>,<jats:italic>R<\/jats:italic>)\u20101\u2032\u2010OH\u2010BF.<\/jats:p>","DOI":"10.1002\/jssc.201100464","type":"journal-article","created":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T10:18:51Z","timestamp":1316168331000},"page":"3578-3586","source":"Crossref","is-referenced-by-count":7,"title":["Stereoselective liquid chromatographic determination of 1\u2032\u2010oxobufuralol and 1\u2032\u2010hydroxybufuralol in rat liver microsomal fraction using hollow\u2010fiber liquid\u2010phase microextraction for sample preparation"],"prefix":"10.1002","volume":"34","author":[{"given":"Thiago","family":"Barth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo A.","family":"Sim\u00f5es","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f4nica T.","family":"Pupo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura T.","family":"Okano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierina S.","family":"Bonato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,16]]},"reference":[{"volume-title":"Merck Index","year":"2001","author":"O'Neil M. 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