{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:23:02Z","timestamp":1761895382909,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T00:00:00Z","timestamp":1320624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Major Program","award":["2009ZX09502-022"],"award-info":[{"award-number":["2009ZX09502-022"]}]},{"name":"Industry Research Program of Traditional Chinese Medicine","award":["200807020"],"award-info":[{"award-number":["200807020"]}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["3097394","81073043","81173571"],"award-info":[{"award-number":["3097394","81073043","81173571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fingerprints of artificial <jats:italic>Calculus bovis<\/jats:italic> extracts from different solvents were established by ultra\u2010performance liquid chromatography (UPLC) and the anti\u2010bacterial activities of artificial <jats:italic>C. bovis<\/jats:italic> extracts on <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S. aureus<\/jats:italic>) growth were studied by microcalorimetry. The UPLC fingerprints were evaluated using hierarchical clustering analysis. Some quantitative parameters obtained from the thermogenic curves of <jats:italic>S. aureus<\/jats:italic> growth affected by artificial <jats:italic>C. bovis<\/jats:italic> extracts were analyzed using principal component analysis. The spectrum\u2013effect relationships between UPLC fingerprints and anti\u2010bacterial activities were investigated using multi\u2010linear regression analysis. The results showed that peak 1 (taurocholate sodium), peak 3 (unknown compound), peak 4 (cholic acid), and peak 6 (chenodeoxycholic acid) are more significant than the other peaks with the standard parameter estimate 0.453, \u22120.166, 0.749, 0.025, respectively. So, compounds cholic acid, taurocholate sodium, and chenodeoxycholic acid might be the major anti\u2010bacterial components in artificial <jats:italic>C. bovis<\/jats:italic>. Altogether, this work provides a general model of the combination of UPLC chromatography and anti\u2010bacterial effect to study the spectrum\u2013effect relationships of artificial <jats:italic>C. bovis<\/jats:italic> extracts, which can be used to discover the main anti\u2010bacterial components in artificial <jats:italic>C. bovis<\/jats:italic> or other Chinese herbal medicines with anti\u2010bacterial effects.<\/jats:p>","DOI":"10.1002\/jssc.201100500","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T02:53:00Z","timestamp":1320634380000},"page":"3330-3338","source":"Crossref","is-referenced-by-count":17,"title":["Searching for the main anti\u2010bacterial components in artificial Calculus bovis using UPLC and microcalorimetry coupled with multi\u2010linear regression analysis"],"prefix":"10.1002","volume":"34","author":[{"given":"Qing\u2010Ce","family":"Zang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Jun","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Jie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian\u2010Feng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010He","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,7]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"China Pharmacopoeia Committee Pharmacopoeia of the People's Republic of China 1st Div (2005 edition) China Chemical Industry Press Beijing 2005 pp. 47\u201348."},{"key":"e_1_2_6_3_2","first-page":"604","volume":"32","author":"Cai H. 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