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Therefore, the accurate quantification of ACV is relevant, e.g. for kinetic studies on the production of these \u03b2\u2010lactam antibiotics. However, accurate quantification of ACV is a challenge, because it is an active thiol compound which, upon exposure to air, can easily react with other thiol compounds to form oxidized disulfides. We have found that, during exposure to air, the oxidation of ACV occurs both in aqueous standard solutions as well as in biological samples. Qualitative and quantitative determinations of ACV and the oxidized dimer bis\u2010\u03b4\u2010[<jats:sc>L<\/jats:sc>\u2010\u03b1\u2010aminoadipyl]\u2010<jats:sc>L<\/jats:sc>\u2010cysteinyl\u2010<jats:sc>D<\/jats:sc>\u2010valine have been carried out using ion pair reversed\u2010phase ultra high\u2010performance liquid chromatography, hyphenated with tandem mass spectrometry (IP\u2010RP\u2010UPLC\u2010MS\/MS) as the analytical platform. We show that by application of tris(2\u2010carboxy\u2010ethyl)phosphine hydrochloride (TCEP) as the reducing reagent, the total amount of ACV can be determined, while using maleimide as derivatizing reagent enables to quantify the free reduced form only.<\/jats:p>","DOI":"10.1002\/jssc.201100527","type":"journal-article","created":{"date-parts":[[2012,1,5]],"date-time":"2012-01-05T08:07:40Z","timestamp":1325750860000},"page":"225-230","source":"Crossref","is-referenced-by-count":4,"title":["Determination of \u03b4\u2010[<scp>L<\/scp>\u2010\u03b1\u2010aminoadipyl]\u2010<scp>L<\/scp>\u2010cysteinyl\u2010<scp>D<\/scp>\u2010valine in cell extracts of Penicillium chrysogenum using ion pair\u2010RP\u2010UPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"35","author":[{"given":"Reza Maleki","family":"Seifar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit T.","family":"Deshmukh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph J.","family":"Heijnen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter M.","family":"van Gulik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91242-8"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/07388559209069194"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3270185"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00618-9"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)E0444-U"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.4.973-984.1994"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0967-0661(96)00067-6"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19960720)51:2<168::AID-BIT6>3.0.CO;2-H"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1536(76)80160-X"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06667.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90691-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90205-1"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90197-6"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac071016b"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(03)00232-7"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(02)00594-0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.10.007"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1083"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3380"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22786"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10907"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.09.001"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.02.008"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2009.02.025"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.09.055"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/cber.19350680133"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00008a014"},{"key":"e_1_2_6_29_2","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1093\/clinchem\/47.10.1821","volume":"47","author":"Krijt J.","year":"2001","journal-title":"Clin. 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