{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:31:04Z","timestamp":1760920264801,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2011,10,4]],"date-time":"2011-10-04T00:00:00Z","timestamp":1317686400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enantioselective determination of aspartic acid (Asp) in the pineal gland of rodents with various melatonin contents was performed using a highly sensitive and selective two\u2010dimensional HPLC system. After derivatization of the amino group with 4\u2010fluoro\u20107\u2010nitro\u20102,1,3\u2010benzoxadiazole (NBD\u2010F), NBD\u2010Asp was separated using a capillary monolithic ODS column in the first dimension. The fraction of NBD\u2010Asp was automatically collected and transferred to the second dimension, and the <jats:sc>D<\/jats:sc>\u2010 and <jats:sc>L<\/jats:sc>\u2010Asp were separated and determined using a narrowbore enantioselective column. Large amounts of <jats:sc>D<\/jats:sc>\u2010Asp were observed in the pineal gland of the rats and specific strains of mice (C3H and CBA) possessing a high concentration of melatonin in their pineal gland. On the other hand, the amounts of <jats:sc>D<\/jats:sc>\u2010Asp were small in the pineal gland of mice possessing a trace or no melatonin in their pineal gland (ddY, ICR, C57BL and BALB\/c). In other tissues and physiological fluids, no significant strain\u2010dependent changes of the <jats:sc>D<\/jats:sc>\u2010Asp amounts were observed. These results indicate that large amounts of <jats:sc>D<\/jats:sc>\u2010Asp are present only in the pineal gland containing large amounts of melatonin, and special care should be taken when selecting mouse strains for the investigation of <jats:sc>D<\/jats:sc>\u2010Asp.<\/jats:p>","DOI":"10.1002\/jssc.201100609","type":"journal-article","created":{"date-parts":[[2011,10,4]],"date-time":"2011-10-04T06:25:02Z","timestamp":1317709502000},"page":"2847-2853","source":"Crossref","is-referenced-by-count":16,"title":["Enantioselective micro\u20102D\u2010HPLC determination of aspartic acid in the pineal glands of rodents with various melatonin contents"],"prefix":"10.1002","volume":"34","author":[{"given":"Hai","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurika","family":"Miyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsubasa","family":"Oyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Konishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Mita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Hamase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00690-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(86)80329-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8971"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00840-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2006.08.005"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00321-3"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-006-0354-6"},{"journal-title":"J. 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