{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T14:59:56Z","timestamp":1774364396134,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"10-11","license":[{"start":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T00:00:00Z","timestamp":1340668800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>A confirmatory high\u2010pressure liquid chromatographic method for the determination of nine tetracyclines in <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>parus aurata<\/jats:italic> (gilthead seabream) muscle tissue is developed and presented herein. Tetracycline, 4\u2010epi\u2010tetracycline, oxytetracycline, 4\u2010epi\u2010oxytetracycline, chlortetracycline, 4\u2010epi\u2010chlortetracycline, doxycycline, methacycline and demeclocycline were separated on a <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>romasil, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>18 (250 mm \u00d7 4 mm, 5 \u03bcm) analytical column by gradient elution with a mobile phase consisting of 0.001 M ethylenediaminetetraacetic acid\/sodium salt and acetonitrile at 25\u00b0C. Diode array detection with monitoring at 280 nm (for the determination of chlortetracycline, 4\u2010epi\u2010chlortetracycline, methacycline and demeclocycline) and 355 nm (for tetracycline, 4\u2010epi\u2010tetracycline, oxytetracycline, 4\u2010epi\u2010oxytetracycline and demeclocycline) was applied for peak identification and quantification of analytes. Examined antibiotics were isolated from gilthead seabream tissue by leaching using a citrate buffer (pH 4.0) and purified by solid phase extraction using <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>asis <jats:styled-content style=\"fixed-case\">HLB<\/jats:styled-content>(200 mg\/6 mL) cartridges. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>uropean <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>nion <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>ecision 2002\/657\/<jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/jssc.201100996","type":"journal-article","created":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T11:28:03Z","timestamp":1340710083000},"page":"1372-1378","source":"Crossref","is-referenced-by-count":11,"title":["Confirmatory development and validation of <scp>HPLC\u2010DAD<\/scp>method for the determination of tetracyclines in gilthead seabream (<i><scp>S<\/scp>parus aurata<\/i>) muscle tissue"],"prefix":"10.1002","volume":"35","author":[{"given":"Evaggelia N.","family":"Evaggelopoulou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria F.","family":"Samanidou","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Chemistry Department of Chemistry Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700252"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600137"},{"key":"e_1_2_6_4_1","first-page":"1","article-title":"(EU) No 37\/2010 of 22 December 2009","volume":"15","author":"Commission Regulation","year":"2010","journal-title":"Off. 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