{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:12:37Z","timestamp":1761513157456,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education and Science of Republic of Serbia","award":["172052"],"award-info":[{"award-number":["172052"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>In this paper, the retention prediction models for mixture of \u03b2\u2010lactam antibiotics analyzed by hydrophilic interaction chromatography (<jats:styled-content style=\"fixed-case\">HILIC<\/jats:styled-content>) are presented. The aim of the study was to investigate the retention behavior of some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, and cefuroxime axetil) and penicillins (ampicillin and amoxicillin). Retention of substances with acidic functional group in <jats:styled-content style=\"fixed-case\">HILIC<\/jats:styled-content> is considered to be interesting since the majority of publications in literature are related to basic compounds. In the beginning of the study, classical silica columns were chosen for the retention analysis. Then, preliminary study was done and factors with the most significant influence on the retention factors were selected. These factors with the impact on the retention factors were investigated employing <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ox\u2013<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ehnken design as a tool. On the basis of the obtained results the mathematical models were created and tested using <jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content> test and finally verified. This approach enables the presentation of chromatographic retention in many ways (three\u2010dimensional (3\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>) graphs and simple two\u2010dimensional graphical presentations). All of these gave the possibility to predict the chromatographic retention under different conditions. Furthermore, regarding the structure of the analyzed compounds, the potential retention mechanisms in <jats:styled-content style=\"fixed-case\">HILIC<\/jats:styled-content> were suggested.<\/jats:p>","DOI":"10.1002\/jssc.201200099","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T07:41:12Z","timestamp":1340869272000},"page":"1424-1431","source":"Crossref","is-referenced-by-count":12,"title":["Assessment of \u03b2\u2010lactams retention in hydrophilic interaction chromatography applying <scp>B<\/scp>ox\u2013<scp>B<\/scp>ehnken <scp>D<\/scp>esign"],"prefix":"10.1002","volume":"35","author":[{"given":"Marko","family":"Jovanovi\u0107","sequence":"first","affiliation":[{"name":"University of Belgrade Department of Drug Analysis Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tijana","family":"Raki\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade Department of Drug Analysis Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biljana","family":"Jan\u010di\u0107\u2010Stojanovi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade Department of Drug Analysis Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An\u0111elija","family":"Malenovi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade Department of Drug Analysis Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darko","family":"Ivanovi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade Department of Drug Analysis Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirjana","family":"Medenica","sequence":"additional","affiliation":[{"name":"University of Belgrade Department of Physical Chemistry and Instrumental Methods Faculty of Pharmacy  Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)96972-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)81096-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600199"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.03.011"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.06.009"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.02.071"},{"key":"e_1_2_6_8_1","first-page":"1010","volume":"22","author":"Grumbach E. 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