{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:44:45Z","timestamp":1792608285742,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T00:00:00Z","timestamp":1341187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>An efficient and environmental friendly ionic liquid based dispersive liquid\u2013liquid microextraction procedure was optimized for determination of rifaximin in rat serum by reverse phase high\u2010performance liquid chromatography. The effect of ionic liquids, dispersive solvents, extractant\/disperser ratio, and salt concentrations on sample recovery and enrichment factors were studied. Among the five ionic liquids studied in the present investigation, 1\u2010butyl\u20103\u2010methylimidazolium hexafluorophosphate was found to be most effective for extraction of rifaximin. The recovery was found to be more than 98% using 1\u2010butyl\u20103\u2010methylimidazolium hexafluorophosphate and methanol as extraction and dispersive solvents, at an extractant\/disperser ratio of 0.43. The recovery was further enhanced to 99.5% by the addition of 5.0% NaCl solution. A threefold enhancement in detection limit was achieved when compared to protein precipitation. The ionic liquid containing the extracted rifaximin was directly injected into HPLC system. The linear relationship was observed in the range of 0.03\u201310.0 \u03bcg\/mL with the correlation coefficient (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>) 0.9998. Limits of detection and quantification were found to be 0.01 and 0.03 \u03bcg\/mL, respectively. The relative standard deviation was 2.5%. The method was validated and applied to study pharmacokinetics of rifaxmin in rat serum.<\/jats:p>","DOI":"10.1002\/jssc.201200202","type":"journal-article","created":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T06:17:29Z","timestamp":1341209849000},"page":"1945-1952","source":"Crossref","is-referenced-by-count":27,"title":["Determination of rifaximin in rat serum by ionic liquid based dispersive liquid\u2013liquid microextraction combined with <scp>RP<\/scp>\u2010<scp>HPLC<\/scp>"],"prefix":"10.1002","volume":"35","author":[{"given":"Ramisetti","family":"Nageswara Rao","sequence":"first","affiliation":[{"name":"Analytical Chemistry Division, D\u2010215, Discovery Laboratory Indian Institute of Chemical Technology  Tarnaka Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajavarapu","family":"Mastan Vali","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Division, D\u2010215, Discovery Laboratory Indian Institute of Chemical Technology  Tarnaka Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alamanda","family":"Vara Prasada Rao","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry Andhra University  Visakhapatnam India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300127-MCP200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1135"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.11.061"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(95)00075-T"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.05.013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.10.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-004-2894-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.02.009"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.03.007"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.05.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.11.088"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.065"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.12.091"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.08.017"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac069394o"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/b500865b"},{"key":"e_1_2_7_18_1","first-page":"1758","volume":"1216","author":"Liu W.","year":"2009","journal-title":"J. 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