{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:14:33Z","timestamp":1761743673470,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T00:00:00Z","timestamp":1346716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Liquid chromatographic separation of stereoisomers of darunavir on <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hiralpak <jats:styled-content style=\"fixed-case\">AD\u2010H<\/jats:styled-content>, a column containing the stationary phase coated with amylose tris(3,5\u2010dimethylphenylcarbamate) as a chiral selector, was studied under normal\u2010phase conditions at different temperatures between 20 and 50\u00b0C. The effect of quality and quantity of different polar organic modifiers viz: methanol, ethanol, 1\u2010propanol, and 2\u2010propanol in the mobile phase as well as column temperature on retention, separation, and resolution was investigated and optimized. The optimum separation was accomplished using a mobile phase composed of <jats:italic>n<\/jats:italic>\u2010hexane\/ethanol\/diethyl amine (80:20:0.1 v\/v\/v) at 40\u00b0C. Apparent thermodynamic parameters \u0394<jats:italic>H<\/jats:italic><jats:sup>0<\/jats:sup> and \u0394<jats:italic>S<\/jats:italic>* were derived from the <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>an't <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>off plots (ln<jats:italic>k<\/jats:italic>\u2032 versus 1\/<jats:italic>T<\/jats:italic>) and used to explain the strength of interactions between the stereoisomers and amylose tris(3,5\u2010dimethylphenylcarbamate) coated chiral stationary phase.<\/jats:p>","DOI":"10.1002\/jssc.201200410","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T06:21:43Z","timestamp":1346739703000},"page":"2671-2677","source":"Crossref","is-referenced-by-count":14,"title":["Liquid chromatographic separation and thermodynamic investigation of stereoisomers of darunavir on <scp>C<\/scp>hiralpak <scp>AD\u2010H<\/scp>column"],"prefix":"10.1002","volume":"35","author":[{"given":"Ramisetti 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