{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:43:51Z","timestamp":1788871431078,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T00:00:00Z","timestamp":1351555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Faculty of Medicine - University of S\u00e3o Paulo","award":["LIM 40\/HC-FMUSP"],"award-info":[{"award-number":["LIM 40\/HC-FMUSP"]}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2009\/08314-9"],"award-info":[{"award-number":["2009\/08314-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["470643\/2009-9"],"award-info":[{"award-number":["470643\/2009-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Here, we present a method for measuring barbiturates (butalbital, secobarbital, pentobarbital, and phenobarbital) in whole blood samples. To accomplish these measurements, analytes were extracted by means of hollow\u2010fiber liquid\u2010phase microextraction in the three\u2010phase mode. Hollow\u2010fiber pores were filled with decanol, and a solution of sodium hydroxide (pH 13) was introduced into the lumen of the fiber (acceptor phase). The fiber was submersed in the acidified blood sample, and the system was subjected to an ultrasonic bath. After a 5 min extraction, the acceptor phase was withdrawn from the fiber and dried under a nitrogen stream. The residue was reconstituted with ethyl acetate and trimethylanilinium hydroxide. An aliquot of 1.0 \u03bcL of this solution was injected into the gas chromatograph\/mass spectrometer, with the derivatization reaction occurring in the hot injector port (flash methylation). The method proved to be simple and rapid, and only a small amount of organic solvent (decanol) was needed for extraction. The detection limit was 0.5 \u03bcg\/mL for all the analyzed barbiturates. The calibration curves were linear over the specified range (1.0 to 10.0 \u03bcg\/mL). This method was successfully applied to postmortem samples (heart blood and femoral blood) collected from three deceased persons previously exposed to barbiturates.<\/jats:p>","DOI":"10.1002\/jssc.201200444","type":"journal-article","created":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T02:41:54Z","timestamp":1351564914000},"page":"3361-3368","source":"Crossref","is-referenced-by-count":25,"title":["Hollow\u2010fiber liquid\u2010phase microextraction and gas chromatography\u2010mass spectrometry of barbiturates in whole blood samples"],"prefix":"10.1002","volume":"35","author":[{"given":"Rafael Almeida","family":"Menck","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di\u00f3genes Saulo","family":"de Lima","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia Carolina","family":"Seulin","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilma","family":"Leyton","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Augusto","family":"Pasqualucci","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of S\u00e3o Paulo  Brazil"},{"name":"Death Verification Service University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Romero","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael David","family":"Osselton","sequence":"additional","affiliation":[{"name":"Department of Forensic &amp; Biological Sciences Bournemouth University  UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Yonamine","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,30]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"457","volume-title":"Goodman and Gilman\u00b4s The Pharmacological Basis of Therapeutics","author":"Mihic S. 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