{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:01:02Z","timestamp":1791086462998,"version":"4.1.0"},"reference-count":143,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,6]],"date-time":"2013-01-06T00:00:00Z","timestamp":1357430400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation of Basic Researches","doi-asserted-by":"crossref","award":["11-03-00829-a"],"award-info":[{"award-number":["11-03-00829-a"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Russian Federal Program \u201cResearch","award":["14.740.11.0382"],"award-info":[{"award-number":["14.740.11.0382"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>The application of monoliths for realization of solid\u2010phase biocatalytic processes was dramatically extended since the beginning of new century. Different enzyme immobilization techniques regarding these modern stationary phases have been developed, adapted, and optimized within last decade. The choice of enzyme immobilization method depends on material nature and monolith manufacturing. The present review collected, analyzed, and discussed the accessible published data on existing approaches and specialties of preparation of flow\u2010through enzyme reactors based on monoliths.<\/jats:p>","DOI":"10.1002\/jssc.201200594","type":"journal-article","created":{"date-parts":[[2013,1,6]],"date-time":"2013-01-06T09:49:07Z","timestamp":1357465747000},"page":"110-127","source":"Crossref","is-referenced-by-count":36,"title":["Flow\u2010through immobilized enzyme reactors based on monoliths: I. Preparation of heterogeneous biocatalysts"],"prefix":"10.1002","volume":"36","author":[{"given":"Evgenia G.","family":"Vlakh","sequence":"first","affiliation":[{"name":"Institute of Macromolecular Compounds Russian Academy of Sciences St. Petersburg Russia"},{"name":"Faculty of Chemistry Saint\u2010Petersburg State University St. Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana B.","family":"Tennikova","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Compounds Russian Academy of Sciences St. Petersburg Russia"},{"name":"Faculty of Chemistry Saint\u2010Petersburg State University St. Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja02262a018"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527607668"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/op0340311"},{"key":"e_1_2_7_5_1","first-page":"75","volume":"39","author":"Guisan J. M.","year":"1991","journal-title":"Biotechnol. Bioeng."},{"key":"e_1_2_7_6_1","volume-title":"Bioanalytical Applications of Enzymes","author":"Seulter C.","year":"1992"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(99)01322-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200700082"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/01483919008051787"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)83340-X"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(96)00545-6"},{"key":"e_1_2_7_12_1","volume-title":"Monolithic Materials: Preparation, Properties and Applications","author":"Svec F.","year":"2003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.090"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.05.036"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80250-G"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19960220)49:4<355::AID-BIT1>3.0.CO;2-O"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500661"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700362"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.08.045"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800641"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.19961100115"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm970204n"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-45345-8_2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2006.01.096"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0614902"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02744835"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200204)23:7\/8<1116::AID-ELPS1116>3.0.CO;2-O"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500225"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700284"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(79)90107-8"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01282-X"},{"key":"e_1_2_7_32_1","first-page":"166","volume":"25","author":"Platonova G. A.","year":"1999","journal-title":"Russ. J. Bioorg. Chem."},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(99)00817-X"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00551-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401800"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.10.006"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.11.006"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500171"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.034"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-582-4_17"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.08.036"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1070427209120179"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2008.06.002"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac049001q"},{"key":"e_1_2_7_45_1","first-page":"166","volume":"25","author":"Platonova G. A.","year":"1999","journal-title":"Russ. J. Bioorg. Chem."},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/21.2.G41"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1557\/PROC-115-229"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.076"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700337"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01029-7"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.06.034"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr049796h"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.01.030"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800596"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0611519"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.12.091"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2010.12.018"},{"key":"e_1_2_7_58_1","volume-title":"Affinity Chromatography","author":"Turkova J.","year":"1978"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/apmc.1978.050700109"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10391"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00105-8"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01055-X"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.584"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1070427208080168"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac061638h"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(02)80014-7"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-053-9"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01027-5"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac980613i"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305841"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500234"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr0502727"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.11.029"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/cc0100060"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.10.047"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.10.046"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.09.100"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9025362"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.12.061"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.11.029"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac702343a"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0504767"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.12.011"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1039\/b920789a"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.12.022"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf200472x"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1745-6"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-008-0353-6"},{"key":"e_1_2_7_89_1","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1016\/S0021-9258(17)30106-0","volume":"54","author":"Bethell G. S.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)85622-8"},{"key":"e_1_2_7_91_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7689747"},{"key":"e_1_2_7_92_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360370305"},{"key":"e_1_2_7_93_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900221"},{"key":"e_1_2_7_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.12.083"},{"key":"e_1_2_7_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.05.080"},{"key":"e_1_2_7_96_1","volume-title":"Immobilized Affinity Ligand Techniques","author":"Hermanson G. T.","year":"1992"},{"key":"e_1_2_7_97_1","first-page":"110.1","volume-title":"The Biomedical Engineering Handbook","author":"Drumheller P. D.","year":"2000"},{"key":"e_1_2_7_98_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0700629"},{"key":"e_1_2_7_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2004.11.071"},{"key":"e_1_2_7_100_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01026430"},{"key":"e_1_2_7_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2004.01.020"},{"key":"e_1_2_7_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2011.03.028"},{"key":"e_1_2_7_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbbm.2006.10.005"},{"key":"e_1_2_7_104_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.197211031"},{"key":"e_1_2_7_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/0032-3861(72)90084-5"},{"key":"e_1_2_7_106_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1500"},{"key":"e_1_2_7_107_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1979"},{"key":"e_1_2_7_108_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.11.102"},{"key":"e_1_2_7_109_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.29130"},{"key":"e_1_2_7_110_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1470-8744.1990.tb00085.x"},{"key":"e_1_2_7_111_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)04083-0"},{"key":"e_1_2_7_112_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90139-0"},{"key":"e_1_2_7_113_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.300080121"},{"key":"e_1_2_7_114_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008117827057"},{"key":"e_1_2_7_115_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990105)62:1<30::AID-BIT4>3.0.CO;2-E"},{"key":"e_1_2_7_116_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020180q"},{"key":"e_1_2_7_117_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr0255452"},{"key":"e_1_2_7_118_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac034108j"},{"key":"e_1_2_7_119_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070705k"},{"key":"e_1_2_7_120_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac8026564"},{"key":"e_1_2_7_121_1","volume-title":"Bioaffinity Chromatography","author":"Turkova J.","year":"1993"},{"key":"e_1_2_7_122_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.01.001"},{"key":"e_1_2_7_123_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.648"},{"key":"e_1_2_7_124_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.23326"},{"key":"e_1_2_7_125_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.32657"},{"key":"e_1_2_7_126_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0100749"},{"key":"e_1_2_7_127_1","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9314(20020101)25:1\/2<3::AID-JSSC3>3.0.CO;2-L"},{"key":"e_1_2_7_128_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0200421"},{"key":"e_1_2_7_129_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac026240"},{"key":"e_1_2_7_130_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie049354f"},{"key":"e_1_2_7_131_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac048388u"},{"key":"e_1_2_7_132_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200700309"},{"key":"e_1_2_7_133_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2008.09.002"},{"key":"e_1_2_7_134_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac035107u"},{"key":"e_1_2_7_135_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm060947l"},{"key":"e_1_2_7_136_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410304"},{"key":"e_1_2_7_137_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200600554"},{"key":"e_1_2_7_138_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-009-0476-8"},{"key":"e_1_2_7_139_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2009.03.002"},{"key":"e_1_2_7_140_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3073"},{"key":"e_1_2_7_141_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000550"},{"key":"e_1_2_7_142_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5501-6"},{"key":"e_1_2_7_143_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-006-0075-y"},{"key":"e_1_2_7_144_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.05.059"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201200594","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201200594","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201200594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:06:26Z","timestamp":1760868386000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201200594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":143,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/jssc.201200594"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201200594","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]}}}