{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:36:14Z","timestamp":1760920574843,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2012,12,7]],"date-time":"2012-12-07T00:00:00Z","timestamp":1354838400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Comiss\u00e3o de Fomento da Investiga\u00e7\u00e3o em Cuidados de Sa\u00fade, Minist\u00e9rio da Sa\u00fade","award":["106\/2007"],"award-info":[{"award-number":["106\/2007"]}]},{"name":"FCT","award":["PIC\/IC\/82957\/2007","SFRH\/BD\/16166\/2004\/5E4M"],"award-info":[{"award-number":["PIC\/IC\/82957\/2007","SFRH\/BD\/16166\/2004\/5E4M"]}]},{"name":"Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Several studies indicate that high levels of homocysteine (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>cy) and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010cysteine (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ys) are independent risk factors for cardiovascular disease. The validation and clinical application of an ultra <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> method for analysis of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>cy and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ys is described. The reported method is simple, sensitive, rapid, precise, and less aggressive than other previously reported methods. The effect of the derivatization reaction time, pH, and organic solvent contents in the mobile phase are described and discussed. Optimized conditions resulted in excellent peak shapes. Results of method validation showed a good linearity (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> \u2265 0.993) over the investigated concentration ranges and were observed for both compounds. The <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">LOQ<\/jats:styled-content> were 0.05 \u03bcM and 0.15 \u03bcM for <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>cy and 0.24 \u03bcM and 0.80 \u03bcM for <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ys, respectively. Validation results proved that the method precision was good and the accuracy was satisfactory. This validated method was successfully applied in an epidemiological study to measure and compare the prevalence of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>cy and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ys high levels in plasma of <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>ortuguese type 2 diabetic patients with and without angiopathy. The study results showed that prevalence of hyperhomocysteinemia and hypercysteinemia were at least two times higher in diabetic patients with angiopathy compared to diabetics without angiopathy.<\/jats:p>","DOI":"10.1002\/jssc.201200672","type":"journal-article","created":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T05:20:01Z","timestamp":1355116801000},"page":"3427-3433","source":"Crossref","is-referenced-by-count":20,"title":["Validation and clinical application of an <scp>UHPLC<\/scp> method for simultaneous analysis of total homocysteine and cysteine in human plasma"],"prefix":"10.1002","volume":"35","author":[{"given":"Ana","family":"Valente","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition National Institute of Health Dr. Ricardo Jorge Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Ros\u00e1rio","family":"Bronze","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy University of Lisbon Lisbon Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Oeiras Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Bicho","sequence":"additional","affiliation":[{"name":"Genetics Laboratory Faculty of Medicine University of Lisbon Lisbon Portugal"},{"name":"Instituto Rocha Cabral Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Duarte","sequence":"additional","affiliation":[{"name":"Portuguese Diabetes Association Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena Soares","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition National Institute of Health Dr. Ricardo Jorge Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.3.183"},{"key":"e_1_2_7_3_1","first-page":"71","volume":"33","author":"Tokg\u00f6z S.","year":"2003","journal-title":"Turk. 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