{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:24:32Z","timestamp":1760919872212,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,28]],"date-time":"2013-01-28T00:00:00Z","timestamp":1359331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p>Iron is an essential element in human development. It is imperative for oxygen and electron transport and also for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and neurotransmitters synthesis. On the other hand, this metal is able to participate in Fenton's reaction that in turn leads to free radical damage. The most toxic fraction of iron \u2013 nontransferrin\u2010bound iron and its part desferrioxamine\u2010chelatable iron \u2013 can serve as an exquisite biomarker in the identification of iron imbalance. The goal of the present study was to devise a simple, repeatable, and inexpensive method for the determination of desferrioxamine\u2010chelatable iron in serum blood samples. The assay procedure is based on desferrioxamine complex formation with iron ions followed to ferrioxamine and its quantitative measurement using <jats:styled-content style=\"fixed-case\">RP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> method. The desferrioxamine\u2010chelatable iron was extracted from blood by centrifugation and <jats:styled-content style=\"fixed-case\">SPE<\/jats:styled-content> method. Chromatographic separation was performed at 40\u00b0C by step\u2010form gradient elution using <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>adenza <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>18 column (150 \u00d7 4.6 mm id, particle size of 3.0 \u03bcm) connected with precolumn for contaminants removal. Gradient <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> elution has been carried out with solvent A (10 mM Tris\u2010HCl, pH 5.5) and solvent B (<jats:styled-content style=\"fixed-case\">ACN<\/jats:styled-content>). The flow rate was 1.2 mL\/min, and the total separation time was 5 min. The linear quantitation range was 2.5\u2013500 \u03bcM (<jats:italic>r<\/jats:italic> = 0.9973), and the <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">LOQ<\/jats:styled-content> were 0.42 and 1.29 \u03bcM, respectively. Proposed <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> method allowed for the determination of desferrioxamine\u2010chelatable iron fraction's of nontransferrin\u2010bound iron, both in the buffer and the serum supplemented with iron ions as well as in the patients\u2019 serum samples with good results of precision and recovery. The developed method found to be sufficiently precise and reproducible for established conditions and after validation and may be used for routine assay of desferrioxamine\u2010chelatable iron in biological samples.<\/jats:p>","DOI":"10.1002\/jssc.201200683","type":"journal-article","created":{"date-parts":[[2012,11,6]],"date-time":"2012-11-06T04:36:52Z","timestamp":1352176612000},"page":"665-669","source":"Crossref","is-referenced-by-count":4,"title":["Ability to determine the desferrioxamine\u2010chelatable iron fractions of nontransferrin\u2010bound iron using <scp>HPLC<\/scp>"],"prefix":"10.1002","volume":"36","author":[{"given":"Marcin","family":"Koba","sequence":"first","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy Collegium Medicum of Nicolaus Copernicus University Bydgoszcz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur","family":"S\u0142omka","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Pharmacy Collegium Medicum of Nicolaus Copernicus University Bydgoszcz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"B\u0105czek","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Medical University of Gda\u0144sk Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142 P.","family":"Marsza\u0142\u0142","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Faculty of Pharmacy Collegium Medicum of Nicolaus Copernicus University Bydgoszcz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"\u017bekanowska","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Pharmacy Collegium Medicum of Nicolaus Copernicus University Bydgoszcz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu3030283"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2431-11-62"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/945289"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"280","DOI":"10.2174\/157341211797458014","volume":"7","author":"S\u0142omka A.","year":"2011","journal-title":"Curr. 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