{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:40:00Z","timestamp":1787892000492,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2013,6,7]],"date-time":"2013-06-07T00:00:00Z","timestamp":1370563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>A broad forensic screening method for 256 analytes in whole blood based on a fully automated <jats:styled-content style=\"fixed-case\">SPE<\/jats:styled-content> robotic extraction and ultra\u2010high\u2010performance liquid chromatography (UHPLC) with <jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> with data\u2010independent acquisition has been developed. The limit of identification was evaluated for all 256 compounds and 95 of these compounds were validated with regard to matrix effects, extraction recovery, and process efficiency. The limit of identification ranged from 0.001 to 0.1\u00a0mg\/kg, and the process efficiency exceeded 50% for 73 of the 95 analytes. As an example of application, 1335 forensic traffic cases were analyzed with the presented screening method. Of these, 992 cases (74%) were positive for one or more traffic\u2010relevant drugs above the <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>anish legal limits. Commonly abused drugs such as amphetamine, cocaine, and frequent types of benzodiazepines were the major findings. Nineteen less frequently encountered drugs were detected e.g. buprenorphine, butylone, cathine, fentanyl, lysergic acid diethylamide, <jats:italic>m<\/jats:italic>\u2010chlorophenylpiperazine, 3,4\u2010methylenedioxypyrovalerone, mephedrone, 4\u2010methylamphetamine, <jats:italic>p<\/jats:italic>\u2010fluoroamphetamine, and <jats:italic>p<\/jats:italic>\u2010methoxy\u2010<jats:italic>N<\/jats:italic>\u2010methylamphetamine. In conclusion, using <jats:styled-content style=\"fixed-case\">UHPLC<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> screening with data\u2010independent acquisition resulted in the detection of common drugs of abuse as well as new designer drugs and more rarely occurring drugs. Thus, <jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> screening of blood samples constitutes a practical way for screening traffic cases, with the exception of \u03b4\u20109\u2010tetrahydrocannabinol, which should be handled in a separate method.<\/jats:p>","DOI":"10.1002\/jssc.201200921","type":"journal-article","created":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T13:33:47Z","timestamp":1366637627000},"page":"2081-2089","source":"Crossref","is-referenced-by-count":76,"title":["Screening for illicit and medicinal drugs in whole blood using fully automated <scp>SPE<\/scp> and ultra\u2010high\u2010performance liquid chromatography with <scp>TOF<\/scp>\u2010<scp>MS<\/scp> with data\u2010independent acquisition"],"prefix":"10.1002","volume":"36","author":[{"given":"Anders Just","family":"Pedersen","sequence":"first","affiliation":[{"name":"Section of Forensic Chemistry Department of 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