{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:09:01Z","timestamp":1775236141411,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,3,8]],"date-time":"2013-03-08T00:00:00Z","timestamp":1362700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Director General, Central Council for Research in Ayurvedic Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>Ursolic acid (<jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>) is the most important bioactive phytoconstituent of <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>ucalyptus<\/jats:italic> \u00d7 <jats:italic>hybrida<\/jats:italic> <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>aiden leaves and exhibits anticancer, antimutagenic, anti\u2010inflammatory, antioxidative, and antiprotozoal activities. In this study, microwave\u2010assisted extraction technique was employed for rapid isolation of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> from the leaves of <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>ucalyptus<\/jats:italic> \u00d7 <jats:italic>hybrida<\/jats:italic> and simultaneously <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2010diode array method was developed for the quantification of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>. Effects of several experimental parameters on the extraction efficiencies of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>, such as type and volume of extraction solvents, microwave power and extraction time, were evaluated. The optimal extraction conditions were found to be 20 mL of a mixture of chloroform\/methanol, 60:40; liquid\u2010to\u2010material ratio, 4:1; preleaching time, 10 min; microwave power, 600 W; temperature, 50\u00b0C; and microwave irradiation time, 5 min. Under the optimum conditions, the yield of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> was found to be 1.95 \u00b1 0.08% in the dry leaves of <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>ucalyptus<\/jats:italic> \u00d7 <jats:italic>hybrida<\/jats:italic>. The results showed that microwave\u2010assisted extraction is a more rapid extraction method with higher yield and lower solvent consumptions than the conventional method. It is a faster, convenient, and appropriate method and it may be used for rapid isolation and quantification of <jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content> and other important phytoconstituents present in the leaves of <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>ucalyptus<\/jats:italic> \u00d7 <jats:italic>hybrida<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201200950","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T06:39:21Z","timestamp":1358836761000},"page":"1255-1262","source":"Crossref","is-referenced-by-count":16,"title":["Microwave\u2010assisted extraction and rapid isolation of ursolic acid from the leaves of <i><scp>E<\/scp>ucalyptus<\/i> \u00d7 <i>hybrida<\/i><scp>M<\/scp>aiden and its quantification using <scp>HPLC<\/scp>\u2010diode array technique"],"prefix":"10.1002","volume":"36","author":[{"given":"Subash C.","family":"Verma","sequence":"first","affiliation":[{"name":"Central Council for Research in Ayurvedic Sciences Janakpuri New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chhoten L.","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Chemistry M. 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