{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:06:21Z","timestamp":1791093981513,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T00:00:00Z","timestamp":1363132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"crossref","award":["0315333B"],"award-info":[{"award-number":["0315333B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>Lectin\u2010functionalized monolithic columns were prepared within polyether ether ketone (PEEK) columns (150 \u00d7 4.6 mm id) via transition metal\u2010catalyzed ring\u2010opening metathesis polymerization of norborn\u20102\u2010ene (<jats:styled-content style=\"fixed-case\">NBE<\/jats:styled-content>) and trimethylolpropane\u2010tris(5\u2010norbornene\u20102\u2010carboxylate) (<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>) using the first\u2010generation <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>rubbs initiator <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>u<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>(<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>y<jats:sub>3<\/jats:sub>)<jats:sub>2<\/jats:sub>(<jats:styled-content style=\"fixed-case\">CHP<\/jats:styled-content>h) (1, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>y = cyclohexyl) in the presence of a macro\u2010 and microporogen, i.e. of 2\u2010propanol and toluene. Postsynthesis functionalization was accomplished via in situ grafting of 2,5\u2010dioxopyrrolidin\u20101\u2010yl\u2010bicyclo[2.2.1]hept\u20105\u2010ene\u20102\u2010carboxylate to the surface of the monoliths followed by reaction with \u03b1,\u03c9\u2010diamino\u2010poly(ethyleneglycol). The pore structure of the poly(ethyleneglycol)\u2010 derivatized monoliths was investigated by electron microscopy and inverse\u2010size exclusion chromatography, respectively. The amino\u2010poly(ethyleneglycol) functionalized monolithic columns were then successfully used for the immobilization of lectin from <jats:italic>Lens culinaris<\/jats:italic> hemagglutinin. The thus prepared lectin\u2010functionalized monoliths were applied to the affinity chromatography\u2010based purification of glucose oxidase. The binding capacity of <jats:italic>Lens culinaris<\/jats:italic> hemagglutinin\u2010immobilized monolithic column for glucose oxidase was found to be 2.2 mg<jats:bold>\/<\/jats:bold>column.<\/jats:p>","DOI":"10.1002\/jssc.201201042","type":"journal-article","created":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T06:27:27Z","timestamp":1357540047000},"page":"1169-1175","source":"Crossref","is-referenced-by-count":22,"title":["Ring\u2010opening metathesis polymerization\u2010derived, lectin\u2010functionalized monolithic supports for affinity separation of glycoproteins"],"prefix":"10.1002","volume":"36","author":[{"given":"Rajendar","family":"Bandari","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Makromolekulare Stoffe und Faserchemie Institut f\u00fcr Polymerchemie Universit\u00e4t Stuttgart  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Kuballa","sequence":"additional","affiliation":[{"name":"Galab 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