{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:28:23Z","timestamp":1760920103814,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2013,6,18]],"date-time":"2013-06-18T00:00:00Z","timestamp":1371513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Twelfth Five-Year National Science & Technology Support Program","award":["2012BAI29B06"],"award-info":[{"award-number":["2012BAI29B06"]}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["KSCX2-YW-R-166"],"award-info":[{"award-number":["KSCX2-YW-R-166"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science & Technology Major Project \u201cKey New Drug Creation and Manufacturing Program","award":["2011ZX09307-002-03"],"award-info":[{"award-number":["2011ZX09307-002-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>A rapid and convenient method was established to preparatively isolate the three ellagic acid types of compounds, which were the main polyphenols in <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>uphorbia pekinensis<\/jats:italic>, by flexibly applying solvent extraction combined with counter\u2010current chromatography (<jats:styled-content style=\"fixed-case\">CCC<\/jats:styled-content>). The total extract (extracted using 95% ethanol) of <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content><\/jats:italic>. <jats:italic>pekinensis<\/jats:italic> was pretreated by two simple steps before <jats:styled-content style=\"fixed-case\">CCC<\/jats:styled-content> isolation, following the procedure: the total extract was extracted by classical solvent extraction using petroleum ether and ethyl acetate, respectively, and then the ethyl acetate extract was suspended using 95% ethanol, after being allowed to stand overnight, the sediment was obtained. Partial sediment (100 mg) was then directly separated by <jats:styled-content style=\"fixed-case\">CCC<\/jats:styled-content> with a two\u2010phase solvent system composed of chloroform\u201095% ethanol\u2010water\u201085% formic acid (50:50:50:5, v\/v\/v\/v). About 22 mg of 3,3\u2032\u2010dimethoxy ellagic acid (1), 12 mg of 3,3\u2032\u2010di\u2010<jats:italic><jats:styled-content style=\"fixed-case\">O<\/jats:styled-content><\/jats:italic>\u2010methyl\u20104\u2010<jats:italic><jats:styled-content style=\"fixed-case\">O<\/jats:styled-content><\/jats:italic>\u2010(<jats:italic>\u03b2<\/jats:italic>\u2010<jats:sc>d<\/jats:sc>\u2010xylopyranosyl)ellagic acid (2), and 35 mg of ellagic acid (3) with purities of 96.0, 95.2, and 95.4% were obtained respectively in one step within 4 h. After being purified by washing with methanol, the purities of the three compounds obtained were all above 98%. The purities were determined by <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> and their chemical structures were further identified by <jats:sup>1<\/jats:sup><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> and <jats:sup>13<\/jats:sup><jats:styled-content style=\"fixed-case\">C NMR<\/jats:styled-content> spectroscopy. The recoveries were calculated as 84.6, 85.7, and 89.5%, respectively. The result demonstrated that the present isolation method was rapid, economical and efficient for the preparative separation of polyphenols from <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>. pekinensis<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201300008","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T05:34:32Z","timestamp":1367300072000},"page":"2366-2372","source":"Crossref","is-referenced-by-count":3,"title":["A rapid and convenient method for preparative separation of three indissolvable polyphenols from <i><scp>E<\/scp>uphorbia pekinensis<\/i> by the flexible application of solvent extraction combined with counter\u2010current chromatography"],"prefix":"10.1002","volume":"36","author":[{"given":"Jiamin","family":"Gu","sequence":"first","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai P. R. China"},{"name":"Shanghai University of Traditional Chinese Medicine  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reihong","family":"Feng","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai P. R. 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