{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:12:13Z","timestamp":1761059533620,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2013,6,21]],"date-time":"2013-06-21T00:00:00Z","timestamp":1371772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p><jats:italic>Vitex trifolia<\/jats:italic> L. is an important Indian medicinal plant with diverse pharmacological properties. In a recent study, we reported the isolation and antitubercular activity evaluation of three new diterpenoids from its leaves; here we have developed a validated rapid, simple, precise, and accurate high\u2010performance <jats:styled-content style=\"fixed-case\">TLC<\/jats:styled-content> method for the simultaneous quantification of isolated diterpenoids in <jats:italic>V. trifolia<\/jats:italic>. Diterpenoids, 6\u03b1,7\u03b1\u2010diacetoxy\u201013\u2010hydroxy\u20108(9),14\u2010labdadien (<jats:bold>A<\/jats:bold>), 13\u2010hydroxy\u20105(10),14\u2010halimadien\u20106\u2010one (<jats:bold>B<\/jats:bold>), and 9\u2010hydroxy\u201013(14)\u2010labden\u201016,15\u2010olide (<jats:bold>C<\/jats:bold>) were separated on silica gel 60F<jats:sub>254<\/jats:sub> high\u2010performance <jats:styled-content style=\"fixed-case\">TLC<\/jats:styled-content> plates using chloroform\/acetone (98:2, v\/v) as mobile phase. The quantitation of diterpenoids was carried out using densitometric reflection\/absorption mode at 610 nm after postchromatographic derivatization using a vanillin\/sulfuric acid reagent. A precise and accurate quantification can be performed for compounds <jats:bold>A<\/jats:bold> and <jats:bold>B<\/jats:bold> in the linear working concentration range of 333\u20131000 ng\/band and for <jats:bold>C<\/jats:bold> in the range of 670\u20132000 ng\/band with good correlations (<jats:italic>r<\/jats:italic> = 0.9984, 0.9991, and 0.9994, respectively). The method was validated for peak purity, precision, accuracy, robustness, <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">LOQ<\/jats:styled-content>, as per the <jats:styled-content style=\"fixed-case\">ICH<\/jats:styled-content> guidelines. The method reported here is simple, reproducible and may be applied for the quantitative analysis of the above diterpenoids in the leaves of <jats:italic>V. trifolia<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201300131","type":"journal-article","created":{"date-parts":[[2013,5,18]],"date-time":"2013-05-18T17:16:50Z","timestamp":1368897410000},"page":"2373-2378","source":"Crossref","is-referenced-by-count":2,"title":["Validated HPTLC method for the simultaneous quantification of diterpenoids in <i>Vitex<\/i><i>trifolia<\/i> L."],"prefix":"10.1002","volume":"36","author":[{"given":"Neerja","family":"Tiwari","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department CSIR\u2010Central Institute of Medicinal and Aromatic Plants  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhilesh Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department CSIR\u2010Central Institute of Medicinal and Aromatic Plants  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madan Mohan","family":"Gupta","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department CSIR\u2010Central Institute of Medicinal and Aromatic Plants  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,21]]},"reference":[{"volume-title":"Glossary of Indian Medicinal Plants","year":"1956","author":"Chopra R. 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