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The method uses a low\u2010viscosity solution of dextran as a sieving polymer. A commercial coating agent (EOTrol LN) was added to the separation buffer to control the EOF of the chips. The potential of this coating agent to prevent protein adsorption on the walls of the SU\u20108 channels was also evaluated. Additionally, the fluorescence background of the SU\u20108 material was studied to improve the sensitivity of the method. By selecting an excitation wavelength of 532 nm at which the background fluorescence remains low and by replacing the mercury arc lamp by a laser in the detection system, an LOD in the nanomolar range was achieved for proteins derivatized with the fluorogenic reagent Chromeo P540. Finally, the method was applied to the analysis of milk samples, demonstrating the potential of SU\u20108 microchips for the analysis of proteins in complex food samples.<\/jats:p>","DOI":"10.1002\/jssc.201300275","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T03:25:01Z","timestamp":1369884301000},"page":"2530-2537","source":"Crossref","is-referenced-by-count":22,"title":["Development of an SDS\u2010gel electrophoresis method on SU\u20108 microchips for protein separation with LIF detection: Application to the analysis of whey proteins"],"prefix":"10.1002","volume":"36","author":[{"given":"Maria","family":"del Mar Barrios\u2010Romero","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn G.","family":"Crevill\u00e9n","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Carlos","family":"Diez\u2010Masa","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry (CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)80293-4"},{"key":"e_1_2_7_3_1","volume-title":"Microfluidic Lab\u2010on\u2010a\u2010Chip for Chemical and Biological Analysis and Discovery, Chromatographic Science Series","author":"Li P. 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