{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:33:04Z","timestamp":1790519584912,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002772","name":"Cagliari University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002772","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Catholic University of Rome"},{"DOI":"10.13039\/501100003407","name":"MIUR","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Italian National Research Council (CNR)"},{"name":"Regione Sardegna and Nando Peretti Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>During <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">ESI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> analysis of rat submandibular saliva secreted under isoprenaline stimulation, a protein with an experimental [M+H]<jats:sup>1+<\/jats:sup> = 10\u00a0544.24 <jats:italic>m\/z<\/jats:italic> was detected (17.5 \u00b1 0.7 min). The <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> fragmentation pattern, manually investigated, allowed establishing an internal sequence in agreement with a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010derived sequence of an unknown rat protein coded <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>9<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>3 (<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>wiss\u2010<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>rot). To match the experimental <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> fragmentation pattern and protein mass with theoretical data, the removal from the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content> terminus of the signal peptide and from the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> terminus of three amino acid (a.a.) residues (<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rg\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>la\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>al) and the cyclization of the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal glutamine in pyroglutamic had to be supposed, resulting in a mature protein of 90 a.a. <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">ESI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> of the trypsin digest ensured 100% sequence coverage. For the high glutamine content (34\/90 = 37.8%) we propose to name this protein rat gliadoralin <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> 1\u201390. Low amounts of five different isoforms were sporadically detected, which did not significantly change their relative amounts after stimulation. Gliadoralin <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> is substrate for transglutaminase\u20102, having <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ys 60 and different <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ln residues as major determinants for enzyme recognition. <jats:italic>In silico<\/jats:italic> investigation of superior structures evidenced that a small part of the protein adopts an \u03b1\u2010helical fold, whereas large segments are unfolded, suggesting an unordered conformation.<\/jats:p>","DOI":"10.1002\/jssc.201300312","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T12:57:28Z","timestamp":1367499448000},"page":"2848-2861","source":"Crossref","is-referenced-by-count":4,"title":["Top\u2010down <scp>HPLC<\/scp>\u2013<scp>ESI<\/scp>\u2010<scp>MS<\/scp> characterization of rat gliadoralin <scp>A<\/scp>, a new member of the family of rat submandibular gland glutamine\u2010rich proteins and potential substrate of transglutaminase"],"prefix":"10.1002","volume":"36","author":[{"given":"Tiziana","family":"Cabras","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze della Vita e dell'Ambiente Univ. di Cagliari Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Iavarone","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica Facolt\u00e0 di Medicina, Universit\u00e0 Cattolica Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Pirolli","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica Facolt\u00e0 di Medicina, Universit\u00e0 Cattolica Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Cristina De Rosa","sequence":"additional","affiliation":[{"name":"Istituto di Chimica del Riconoscimento Molecolare CNR Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Vitali","sequence":"additional","affiliation":[{"name":"Istituto di Chimica del Riconoscimento Molecolare CNR Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavino","family":"Faa","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Chirurgiche Sezione di Patologia, Univ. di Cagliari Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Cordaro","sequence":"additional","affiliation":[{"name":"Istituto di Clinica Odontoiatrica, Facolt\u00e0 di Medicina Universit\u00e0 Cattolica Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Messana","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e dell'Ambiente Univ. di Cagliari Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rgen","family":"Ekstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, G\u00f6teborg, Sweden and Dipartimento di Scienze Biomediche Univ. di Cagliari Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Castagnola","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biochimica Clinica Facolt\u00e0 di Medicina, Universit\u00e0 Cattolica Roma Italy"},{"name":"Istituto di Chimica del Riconoscimento Molecolare CNR Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1002\/j.0022-0337.2001.65.12.tb03492.x","volume":"65","author":"Tabak L. 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