{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:38:42Z","timestamp":1791725922415,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T00:00:00Z","timestamp":1375401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>A systematic study of the retention behavior of isomeric triacylglycerols (<jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>s) in silver\u2010ion <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> on a <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hrom<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>pher <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ipids column has been performed between 10 to 40\u00b0C using the most widespread hexane\u2010 and dichloromethane\u2010based mobile phases. The randomization of mono\u2010acyl <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> isomers. The mobile phase composition has no influence on the general retention pattern, but significant differences in the retention order of double bond (<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>) positional isomers in hexane and dichloromethane mobile phases are described and compared with the previous literature data. Saturated <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>s with fatty acyl chain length from <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>7:0 to <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>22:0 are partially separated using the hexane mobile phase but not at all with the dichloromethane mobile phase. The hexane mobile phase enables at least partial resolution of <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> regioisomers with up to seven <jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>s, while the resolution of only <jats:styled-content style=\"fixed-case\">ALA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AAL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AAL<\/jats:styled-content>n isomers is achieved with the dichloromethane mobile phase. The effect of temperature differs significantly depending on the mobile phase composition. Retention times of <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>s increase with increasing temperature in the hexane mobile phase, while an opposite effect is observed for the dichloromethane mobile phase.<\/jats:p>","DOI":"10.1002\/jssc.201300550","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T08:11:14Z","timestamp":1373357474000},"page":"2888-2900","source":"Crossref","is-referenced-by-count":20,"title":["Retention behavior of isomeric triacylglycerols in silver\u2010ion <scp>HPLC<\/scp>: <scp>E<\/scp>ffects of mobile phase composition and temperature"],"prefix":"10.1002","volume":"36","author":[{"given":"Miroslav","family":"L\u00edsa","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumen","family":"Denev","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry with Centre of Phytochemistry Bulgarian Academy of Sciences Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Hol\u010dapek","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,2]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Modifying Lipids for Use in Food","author":"Gunstone F. 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