{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:20:21Z","timestamp":1792099221238,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T00:00:00Z","timestamp":1398384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Smoking is considered to be one of the main risk factors for cancer and other diseases and is the second leading cause of death worldwide. As the anti\u2010tobacco legislation implemented in Europe has reduced secondhand smoke exposure levels, analytical methods must be adapted to these new levels. Recent research has demonstrated that cotinine is the best overall discriminator when biomarkers are used to determine whether a person has ongoing exposure to tobacco smoke. This work proposes a sensitive, simple and low\u2010cost method based on solid\u2010phase extraction and liquid chromatography with diode array detection for the assessment of tobacco smoke exposure by cotinine determination in urine. The analytical procedure is simple and fast (20 min) when compared to other similar methods existing in the literature, and it is cheaper than the mass spectrometry techniques usually used to quantify levels in nonsmokers. We obtained a quantification limit of 12.30 \u03bcg\/L and a recovery of over 90%. The linearity ranges used were 12\u2013250 and 250\u20134000 \u03bcg\/L. The method was successfully used to determine cotinine in urine samples collected from different volunteers and is clearly an alternative routine method that allows active and passive smokers to be distinguished.<\/jats:p>","DOI":"10.1002\/jssc.201301259","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T05:35:54Z","timestamp":1395725754000},"page":"1404-1410","source":"Crossref","is-referenced-by-count":7,"title":["High\u2010performance liquid chromatography with diode\u2010array detection cotinine method adapted for the assessment of tobacco smoke exposure"],"prefix":"10.1002","volume":"37","author":[{"given":"M\u00f3nica","family":"Bartolom\u00e9","sequence":"first","affiliation":[{"name":"Environmental Toxicology National Centre for Environmental Health Instituto de Salud Carlos III  Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandrina","family":"Gallego\u2010Pic\u00f3","sequence":"additional","affiliation":[{"name":"Department of Analytical Sciences Faculty of Sciences National University of Distance Education (UNED)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Huetos","sequence":"additional","affiliation":[{"name":"Environmental Toxicology National Centre for Environmental Health Instituto de Salud Carlos III  Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Argelia","family":"Casta\u00f1o","sequence":"additional","affiliation":[{"name":"Environmental Toxicology National Centre for Environmental Health Instituto de Salud Carlos III  Majadahonda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,25]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"WHO Global Report: Mortality Attributable to Tobacco","author":"World Health Organization","year":"2012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.1103680"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2011.02.037"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.1.3"},{"key":"e_1_2_8_6_1","first-page":"29","volume-title":"Nicotine Chemistry, Metabolism, Kinetics and Biomarkers in Handb. Exp. Pharmacol","author":"Benowitz N. L.","year":"2009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ntr\/ntq237"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900575"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.36.040196.003121"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00748a032"},{"key":"e_1_2_8_11_1","first-page":"293","volume":"109","author":"Van Vunakis H.","year":"1993","journal-title":"IARC Sci. Publ."},{"key":"e_1_2_8_12_1","first-page":"734","volume":"64","author":"Stanek A.","year":"2007","journal-title":"Przeglad Lekarski"},{"key":"e_1_2_8_13_1","first-page":"S87","volume":"95","author":"Buacharoen T.","year":"2012","journal-title":"J. Med. Assoc. Thai."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-007-0331-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.3127"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00343-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.02.006"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.09.044"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1581"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.10.005"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.12.012"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2012.02.017"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5661-4"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.06.028"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(90)80168-O"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00306-6"},{"key":"e_1_2_8_27_1","first-page":"823","volume":"61","author":"Oruc E. E.","year":"2006","journal-title":"Pharmazie"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/44.9.535"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-007-0254-2"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2008.07.005"},{"key":"e_1_2_8_31_1","first-page":"256","volume":"80","author":"Bystrowska B.","year":"2009","journal-title":"Ginekol. Pol."},{"key":"e_1_2_8_32_1","first-page":"357","volume":"17","author":"Wen Y. H.","year":"2009","journal-title":"J. Food Drug Anal."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900575"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-4870-1"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.59.295"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.1118890"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwn301"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2012-002034"},{"key":"e_1_2_8_39_1","first-page":"1062","volume":"26","author":"Mutavdzic Pavlovic D.","year":"2007","journal-title":"Trends"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/13547500701421341"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201301259","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201301259","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:10:30Z","timestamp":1760868630000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201301259"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,25]]},"references-count":39,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/jssc.201301259"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201301259","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,25]]}}}