{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T18:35:49Z","timestamp":1768070149151,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T00:00:00Z","timestamp":1401926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>Hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion\u2010pair chromatography for the separation of aminoglycoside antibiotics in recent years. However, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. In this work, a cysteine\u2010based zwitterionic stationary phase (named Click TE\u2010Cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. Click TE\u2010Cys displayed much better selectivity for structurally similar aminoglycosides. The retention behaviors of aminoglycosides were investigated in detail, revealing that low pH (2.7 or 3.0) and high buffer concentration (\u226550 mM) were preferable for achieving good peak shape and selectivity. Effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin C1, gentamicin C2\/C2a, gentamicin C1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. Additionally, spectinomycin and its related impurities were successfully resolved. The results indicated the great potential of the Click TE\u2010Cys column in the separation of aminoglycoside mixtures and related impurities.<\/jats:p>","DOI":"10.1002\/jssc.201400080","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T16:52:59Z","timestamp":1399049579000},"page":"1781-1787","source":"Crossref","is-referenced-by-count":17,"title":["Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE\u2010Cys column"],"prefix":"10.1002","volume":"37","author":[{"given":"Jie","family":"Wei","sequence":"first","affiliation":[{"name":"School of Pharmacy East China University of Science and Technology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aijin","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical Physics Chinese Academy of Sciences  Dalian 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