{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:16:25Z","timestamp":1778904985661,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Operational Program Research and Development for Innovations-European Regional Development Fund","award":["CZ.1.05\/2.1.00\/03.0058"],"award-info":[{"award-number":["CZ.1.05\/2.1.00\/03.0058"]}]},{"name":"Operational Program Education for Competitiveness","award":["CZ.1.07\/2.3.00\/20.0058"],"award-info":[{"award-number":["CZ.1.07\/2.3.00\/20.0058"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>Although there are existing methods for determining estrogen in human bodily fluids including blood plasma and serum, very little information is available regarding estrogen levels in human cerebrospinal fluid (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>), which is critical to assess in studies of neuroprotective functions and diffusion of neuroprotective estrogens across the blood\u2013brain barrier. To address this problem, a liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of four endogenous estrogens (estrone, 17\u03b1\u2010estradiol, 17\u03b2\u2010estradiol, and estriol) in human <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> was developed. An aliquot (300 \u03bcL) of human <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> was bulk derivatized using dansyl chloride in the sample and 10 \u03bcL was directly injected onto a restricted\u2010access media trap column for protein removal. No off\u2010line sample extraction or cleanup was needed. The limits of detection of estrone, 17\u03b1\u2010estradiol, 17\u03b2\u2010estradiol, and estriol were 17, 28, 13, and 30 pg\/mL, respectively, which is in the parts\u2010per\u2010trillion regime. The method was then applied to human <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> collected from ischemic trauma patients. Endogenous estrogens were detected and quantified, demonstrating the effectiveness of this method.<\/jats:p>","DOI":"10.1002\/jssc.201400165","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T13:57:27Z","timestamp":1399989447000},"page":"2010-2017","source":"Crossref","is-referenced-by-count":18,"title":["Bulk derivatization and direct injection of human cerebrospinal fluid for trace\u2010level quantification of endogenous estrogens using trap\u2010and\u2010elute liquid chromatography with tandem mass spectrometry"],"prefix":"10.1002","volume":"37","author":[{"given":"Hui","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Arlington  Arlington TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Papouskova","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry\u2013RCPTM Palacky University  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karel","family":"Lemr","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry\u2013RCPTM Palacky University  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane G.","family":"Wigginton","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Texas Southwestern Medical Center  Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin A.","family":"Schug","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Texas at Arlington  Arlington TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1997.87.5.0724"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00790-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.07.037"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1515"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1210\/endo.142.1.7911","volume":"142","author":"Dubal D. B.","year":"2001","journal-title":"Endocrinology"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2005.07.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2006.04.006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2006.103788"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-005-0835-7"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0404.2000.102001037.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.8.1007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a117245"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.289.20.2651"},{"key":"e_1_2_8_15_1","first-page":"e023","volume":"4","author":"Clark J. H.","year":"2006","journal-title":"Nucl. Recept. Signal."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.08.010"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.01830"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPA65OUUFASOAN"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.12.030"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.11.014"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300595"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.09.39"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.498"},{"key":"e_1_2_8_24_1","first-page":"1","volume":"64","author":"US Department of Health and Human Services. Food and Drug Administration. Center for Drug Evaluation and Research","year":"2001","journal-title":"Fed. Reg."},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.025478"},{"key":"e_1_2_8_26_1","unstructured":"Shimadzu Application News.Direct injection of blood plasma for the determination of Drugs using \u201cCo\u2010sense for BA\u201d (Part 1). No. L285."},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(80)90011-1"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.03.013"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.02.009"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070494j"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djr531"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201100219"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5038-8"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300055"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.06.025"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.11.002"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201400165","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201400165","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201400165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:57:15Z","timestamp":1760867835000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201400165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":35,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1002\/jssc.201400165"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201400165","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7]]}}}