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Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil, and apomorphine hydrochloride, and one drug that is used as opioid analgesic, tramadol hydrochloride, which is quiet widely misused to treat premature ejaculation. The results indicate the importance of considering the structure and p<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub> values of drugs to be separated along with mobile phase composition. A new optimized, rapid, and accurate liquid chromatography method is also established for simultaneous determination of sildenafil citrate, tadalafil, and apomorphine hydrochloride in pharmaceutical preparations and bulk powders. The chromatographic separation of the three pharmaceuticals was achieved on a calixarene column in less than 10\u00a0min using a binary mobile phase of 35% acetonitrile and 65% 50\u00a0mM sodium perchlorate pH2.5 at 1\u00a0mL\/min flow rate. The method was validated for system efficiency, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability, and robustness. Statistical analysis proved that the method enabled reproducible and selective quantification of all three analytes in bulk drugs and in pharmaceutical preparations.<\/jats:p>","DOI":"10.1002\/jssc.201400276","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T21:13:42Z","timestamp":1409346822000},"page":"2814-2824","source":"Crossref","is-referenced-by-count":9,"title":["Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase"],"prefix":"10.1002","volume":"37","author":[{"given":"Hisham","family":"Hashem","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Zagazig University  Egypt"},{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Jazan University  Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel Ehab","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Egyptian International Pharmaceutical Industries Co. \u201cEIPICo\u201d  Tenth of Ramadan City Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Elhenawee","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Zagazig University  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00419a052"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ciuz.19910250405"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.19951070704"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1693(96)05074-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10610279708034936"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3765(19990201)5:2<738::AID-CHEM738>3.0.CO;2-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/a803805h"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01298-5"},{"key":"e_1_2_6_10_1","first-page":"421","volume-title":"Calixarenes","author":"Matthews S. 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