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Our research has demonstrated that\n                    <jats:italic>Erycibe obtusifolia<\/jats:italic>\n                    has xanthine oxidase inhibitory properties. The purpose of this paper is to describe a new strategy based on a combination of multiple mass spectrometric platforms and thin\u2010layer chromatography bioautography for effectively screening the xanthine oxidase inhibitory and antioxidant properties of\n                    <jats:italic>E. obtusifolia<\/jats:italic>\n                    . This strategy was accomplished through the following steps. (i) Separate the extract of\n                    <jats:italic>E. obtusifolia<\/jats:italic>\n                    into fractions by an autopurification system controlled by liquid chromatography with mass spectrometry. (ii) Determine the active fractions of\n                    <jats:italic>E. obtusifolia<\/jats:italic>\n                    by thin\u2010layer chromatography bioautography. (iii) Identify the structure of the main active compounds with the information provided by direct analysis in real time mass spectrometry. (iv) Calculate the IC\n                    <jats:sub>50<\/jats:sub>\n                    value of each compound against xanthine oxidase using high\u2010performance liquid chromatography. Using the caulis of\n                    <jats:italic>E. obtusifolia<\/jats:italic>\n                    as the experimental material, seven target peaks were screened out as xanthine oxidase inhibitors or antioxidants. Our screening strategy allows for rapid analysis of small molecules with almost no sample preparation and can be completed within a week, making it a useful assay to identify unstable compounds and provide the empirical foundation for\n                    <jats:italic>E. obtusifolia<\/jats:italic>\n                    as a natural remedy for gout and oxidative\u2010stress\u2010related diseases.\n                  <\/jats:p>","DOI":"10.1002\/jssc.201400342","type":"journal-article","created":{"date-parts":[[2014,6,3]],"date-time":"2014-06-03T20:38:49Z","timestamp":1401827929000},"page":"2253-2259","source":"Crossref","is-referenced-by-count":23,"title":["Quick identification of xanthine oxidase inhibitor and antioxidant from\n                    <i>Erycibe obtusifolia<\/i>\n                    by a drug discovery platform composed of multiple mass spectrometric platforms and thin\u2010layer chromatography bioautography"],"prefix":"10.1002","volume":"37","author":[{"given":"Zhiyong","family":"Chen","sequence":"first","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese medicines, Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai P. 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