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The approach utilized dual sample injection and valve\u2010mediated column switching and was based upon a single high\u2010performance liquid chromatography gradient pump. The separation consisted of three distinct sequential separation mechanisms, namely, (i) ion\u2010exchange, (ii) mixed\u2010mode interactions under an applied dual gradient (reversed\u2010phase\/ion\u2010exchange), and (iii) hydrophilic interaction chromatography. Upon first injection, the Scherzo SS C<jats:sub>18<\/jats:sub> column (Imtakt) provided resolution of inorganic anions and cations under isocratic conditions, followed by a dual organic\/salt gradient to elute active pharmaceutical ingredients and their respective organic counterions and potential degradants. At the top of the mixed\u2010mode gradient (high acetonitrile content), the mobile phase flow was switched to a preconditioned hydrophilic interaction liquid chromatography column, and the standard\/sample was reinjected for the separation of hydrophilic carbohydrates, some of which are commonly known excipients in drug formulations. The approach afforded reproducible separation and resolution of up to 23 chemically diverse solutes in a single run. The method was applied to investigate the composition of commercial cough syrups (Robitussin\u00ae), allowing resolution and determination of inorganic ions, active pharmaceutical ingredients, excipients, and numerous well\u2010resolved unknown peaks.<\/jats:p>","DOI":"10.1002\/jssc.201400411","type":"journal-article","created":{"date-parts":[[2014,5,31]],"date-time":"2014-05-31T03:19:01Z","timestamp":1401506341000},"page":"2138-2144","source":"Crossref","is-referenced-by-count":28,"title":["Comprehensive analysis of pharmaceutical products using simultaneous mixed\u2010mode (ion\u2010exchange\/reversed\u2010phase) and hydrophilic interaction liquid chromatography"],"prefix":"10.1002","volume":"37","author":[{"given":"Artaches A.","family":"Kazarian","sequence":"first","affiliation":[{"name":"Australian Centre for Research on Separation Science, School of Chemistry University of Tasmania  Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel 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