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In this study, a simple and sensitive analytical method was established for the simultaneous measurement of nine macrolides in human urine by combining a sub\u20103\u00a0\u03bcm superficially porous particle packed column with charged aerosol detection. After thorough investigation of various sample preparation methods, including two liquid\u2013liquid extraction methods and four solid\u2010phase extraction methods, HLB solid\u2010phase extraction was selected and further optimized. Absolute recovery of the optimized sample preparation method ranged from 99.5\u2013110.2%, indicating its very high extraction\/clean\u2010up efficiency. For chromatography, parameters influencing macrolide separation were systematically optimized, and the resulting conditions allowed baseline separation of nine macrolides within 24\u00a0min using a very simple mobile phase. The established method was validated for linearity, limit of detection, limit of quantification, absolute recovery, and precision. Based on its limit of detection (0.025\u20130.100\u00a0\u03bcg\/mL), the method had similar or greater sensitivity than most methods based on electrochemical detection. It was found that the current method was appropriate for application to real human urine samples after drug administration.<\/jats:p>","DOI":"10.1002\/jssc.201400517","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T20:52:58Z","timestamp":1408740778000},"page":"2837-2843","source":"Crossref","is-referenced-by-count":6,"title":["Combination of a sub\u20103\u00a0\u03bcm superficially porous particle packed column with charged aerosol detection for the simple and sensitive measurement of nine macrolides in human urine"],"prefix":"10.1002","volume":"37","author":[{"given":"Shaodong","family":"Jia","sequence":"first","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In Gi","family":"Song","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Min","family":"Jeong","sequence":"additional","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Hill","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongmi","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Won","family":"Kwon","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20189"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.08.020"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00891-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-003-1801-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.200390056"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.04.042"},{"key":"e_1_2_8_8_1","first-page":"1","volume":"2012","author":"Lantider Kassaye B.","year":"2012","journal-title":"ISRN Anal. 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