{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:16:18Z","timestamp":1761059778334,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>A simple and rapid high\u2010performance thin\u2010layer chromatographic method was developed for the separation and determination of six flavonoids (rutin, luteolin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010glucoside, chamaemeloside, apigenin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010glucoside, luteolin, apigenin) and one coumarin, umbelliferone from chamomile plant samples and dietary supplements. The separation was achieved on amino silica stationary phase using dichloromethane\/acetonitrile\/ethyl formate\/glacial acetic acid\/formic acid (11:2.5:3:1.25:1.25 v\/v\/v\/v\/v) as the mobile phase. The quantitation of each compound was carried out using densitometric reflection\/absorption mode at their respective absorbance maxima after postchromatographic derivatization using natural products reagent (1% w\/v methanolic solution of diphenylboric acid\u2010\u03b2\u2010ethylamino ester). The method was validated for specificity, limits of detection and quantification, precision (intra\u2010 and interday) and accuracy. The limits of detection and quantification were found to be in the range from 6\u201318 and 16\u201355\u00a0ng\/band for six flavonoids and one coumarin, respectively. The intra\u2010 and interday precision was found to be &lt;5% RSD and recovery of all the compounds was &gt;90%. The data acquired from high\u2010performance thin\u2010layer chromatography was processed by principal component analysis using XLSTAT statistical software. Application of principal component analysis and agglomerative hierarchial clustering was successfully able to differentiate two chamomiles (German and Roman) and <jats:italic>Chrysanthemum<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201400646","type":"journal-article","created":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T19:29:07Z","timestamp":1406662147000},"page":"2797-2804","source":"Crossref","is-referenced-by-count":20,"title":["Quantitative determination of seven chemical constituents and chemo\u2010type differentiation of chamomiles using high\u2010performance thin\u2010layer chromatography"],"prefix":"10.1002","volume":"37","author":[{"given":"Satyanarayanaraju","family":"Sagi","sequence":"first","affiliation":[{"name":"National Center for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi  MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bharathi","family":"Avula","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi  MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi  MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi  MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikhlas A.","family":"Khan","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi  MS USA"},{"name":"Division of Pharmacognosy Department of BioMolecular Sciences, School of Pharmacy The University of Mississippi  MS USA"},{"name":"Department of Pharmacognosy College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"22","volume-title":"Herbal Medicine,","author":"Weiss R. 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