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The columns used were packed with a stationary phase containing a phenyl ring attached to the silica surface using different types of linkage molecules. As a basic characteristic of the bonded phases, the hydrophobicity and silanol activity (polarity) were investigated. The presence of the polar amino and amide groups in the structure of the bonded ligand strongly influences the polarity of the bonded phase. Columns were compared according to methylene selectivity using a series of benzene homologues and according to their shape and size selectivity using polycyclic aromatic hydrocarbons. The measurements were done using methanol\/water and acetonitrile\/water mobile phases. The presented results show that the presence of polar functional groups in the ligand structure strongly influences the chromatographic properties of the bonded phase.<\/jats:p>","DOI":"10.1002\/jssc.201400764","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T02:45:17Z","timestamp":1411008317000},"page":"3435-3442","source":"Crossref","is-referenced-by-count":29,"title":["Phenyl\u2010bonded stationary phases\u2014The influence of polar functional groups on retention and selectivity in reversed\u2010phase liquid chromatography"],"prefix":"10.1002","volume":"37","author":[{"given":"Szymon","family":"Bocian","sequence":"first","affiliation":[{"name":"Faculty of Chemistry Department of Environmental Chemistry &amp; Bioanalytics Nicolaus Copernicus University  Torun Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogus\u0142aw","family":"Buszewski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Department of Environmental Chemistry &amp; Bioanalytics Nicolaus Copernicus University  Torun Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,20]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"731","volume":"28","author":"Buszewski B.","year":"1983","journal-title":"Chem. 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