{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T01:22:03Z","timestamp":1779326523631,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2014,10,20]],"date-time":"2014-10-20T00:00:00Z","timestamp":1413763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>A separation method using counter current chromatography coupled with an evaporative light\u2010scattering detection system was developed to purify five triterpenoid saponins from the roots of <jats:italic>Bupleurum falcatum<\/jats:italic>. The methanol extract was loaded onto a Diaion\u00ae HP20 column and fractionated by a methanol and water gradient elution. The saikosaponin\u2010enriched fraction was obtained by elution with 100% methanol. The two\u2010phase solvent systems used for separation were composed of chloroform\/methanol\/isopropanol\/water at a volume ratio of 60:60:1:60 and 6:6:1:6. The relationship between the isopropanol ratio of each phase and the partition coefficients of the target compounds was investigated by calculating partition coefficient by high\u2010performance liquid chromatography and measuring the accurate composition of each phase by <jats:sup>1<\/jats:sup>H NMR spectroscopy. Each fraction obtained was collected and dried, which yielded the following five saikosaponins from 700 mg of injected sample: saikosaponin B<jats:sub>1<\/jats:sub> (8.7 mg), saikosaponin A (86 mg), saikosaponin B<jats:sub>3<\/jats:sub> (17 mg), saikosaponin B<jats:sub>2<\/jats:sub> (41 mg), and saikosaponin C (33 mg). Saikosaponin A showed the most potent cytotoxicity against human cancer cells (gastric cancer, AGS cells; breast cancer, MCF\u20107 cells; and hepatoma, HepG2 cells) after 24 h. The IC<jats:sub>50<\/jats:sub> values for the above three cell types were 34.6, 33.3, and 23.4 \u03bcmol\/L, respectively.<\/jats:p>","DOI":"10.1002\/jssc.201400871","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T13:16:52Z","timestamp":1410787012000},"page":"3587-3594","source":"Crossref","is-referenced-by-count":15,"title":["Separation of triterpenoid saponins from the root of <i>Bupleurum falcatum<\/i> by counter current chromatography: The relationship between the partition coefficients and solvent system composition"],"prefix":"10.1002","volume":"37","author":[{"given":"Kyoung Jin","family":"Lee","sequence":"first","affiliation":[{"name":"College of Pharmacy Natural Products Research Institute Seoul National University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei\u2010Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Natural Products Research Institute Seoul National University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omer","family":"Shehzad","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Abdul Wali Khan University  Mardan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Kyoung","family":"Seo","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Global Top 5 Research Program College of Pharmacy Ewha Womans University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeong Shik","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy Natural Products Research Institute Seoul National University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X90000149"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.14.1023"},{"key":"e_1_2_7_4_1","first-page":"2043","volume":"20","author":"Shimaoka A.","year":"1975","journal-title":"J. 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