{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T21:35:00Z","timestamp":1768426500601,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T00:00:00Z","timestamp":1424908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Science and Technology Project of Zhejiang Province","award":["2012F30027"],"award-info":[{"award-number":["2012F30027"]}]},{"name":"the Science and Technology Project of Zhejiang Province","award":["2013F50021"],"award-info":[{"award-number":["2013F50021"]}]},{"name":"The Zhejiang Science and technology project of TCM","award":["2013ZQ002"],"award-info":[{"award-number":["2013ZQ002"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LY12B05004"],"award-info":[{"award-number":["LY12B05004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p><jats:italic>Phellinus igniarius<\/jats:italic> has antibacterial, antiviral, antioxidative, antitumor, and antimutagenic effects. In this study, an integrative pattern recognition approach using principal component analysis and orthogonal partial least squares discriminant analysis was successfully applied for the rapid analysis of natural compounds in traditional Chinese medicine. An ultra high performance liquid chromatography with synapt high\u2010definition mass spectrometry method and MassLynx software was used. This method employed gradient elution to rapidly analyze and characterize chemical constituents and metabolites after the oral administration of a <jats:italic>P. igniarius<\/jats:italic> ethanol extract. There were 24 peaks within 10 min of the analysis time and 20 of these were identified or tentatively characterized on the basis of their fragmentation behaviors. In the S\u2010plot of the orthogonal partial least squares discriminant analysis, 27 ions were extracted to make the serum. Among them, nine absorbed the prototype components and 18 metabolites were identified in vivo. Glucuronidation, oxidation, and methylation were the major metabolic reactions. This study is the first systematic analysis and characterization of the chemical constituents and metabolites in an ethanol extract of <jats:italic>P. igniarius<\/jats:italic>. This method can be applied to the rapid analysis and characterization of constituents in rat serum after the oral administration of other compounds used in traditional Chinese medicines.<\/jats:p>","DOI":"10.1002\/jssc.201401293","type":"journal-article","created":{"date-parts":[[2015,1,20]],"date-time":"2015-01-20T09:09:31Z","timestamp":1421744971000},"page":"1137-1148","source":"Crossref","is-referenced-by-count":14,"title":["Ultra high performance liquid chromatography with synapt high\u2010definition mass spectrometry and a pattern recognition approach to characterize chemical constituents and rat metabolites after the oral administration of <i>Phellinus igniarius<\/i>"],"prefix":"10.1002","volume":"38","author":[{"given":"Yu","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Medicine Zhejiang Academy of Traditional Chinese Medicine  Hangzhou China"},{"name":"Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"National TCM Key Lab of Serum Pharmacochemistry Key Pharmacometabolomics Platform of Chinese Medicines and Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine Zhejiang Academy of Traditional Chinese Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"An","sequence":"additional","affiliation":[{"name":"National TCM Key Lab of Serum Pharmacochemistry Key Pharmacometabolomics Platform of Chinese Medicines and Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"National TCM Key Lab of Serum Pharmacochemistry Key Pharmacometabolomics Platform of Chinese Medicines and Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Shou","sequence":"additional","affiliation":[{"name":"Department of Medicine Zhejiang Academy of Traditional Chinese Medicine  Hangzhou China"},{"name":"Department of Chemistry Xixi Campus Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1250542"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813142106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200793"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1an15752c"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2370"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0138"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200791"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043990"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200887"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.12.034"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.12.013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.05.048"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.06.017"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2407"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2AN36382H"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2an35780a"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1mb05366c"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1615\/IntJMedMushr.v16.i3.80"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2711-3"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.08.061"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.11.033"},{"key":"e_1_2_8_23_1","first-page":"132","article-title":"Rev. Iberoam","volume":"29","author":"Ayala\u2010Zavala J. F.","year":"2012","journal-title":"Micol."},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2011.2"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/np100216k"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.11.024"},{"key":"e_1_2_8_27_1","first-page":"38","volume":"37","author":"Yu Z. M.","year":"2014","journal-title":"J. Chinese Med. Mater."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3an36902a"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.05.042"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.07.037"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf401691y"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201401293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201401293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:13:46Z","timestamp":1760872426000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201401293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,26]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1002\/jssc.201401293"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201401293","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,26]]}}}