{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:34:22Z","timestamp":1761896062324,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2015,6,8]],"date-time":"2015-06-08T00:00:00Z","timestamp":1433721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Foundation of National Natural Science of China","doi-asserted-by":"crossref","award":["81102162"],"award-info":[{"award-number":["81102162"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>Enterovirus 71 and Coxsackievirus A16 are the main pathogens causing hand\u2010foot\u2010mouth disease. In this paper, microchip capillary electrophoresis with laser\u2010induced fluorescence combined with one\u2010step duplex reverse transcript\u2010polymerase chain reaction has been developed for the detection of Enterovirus 71 and Coxsackievirus A16 in throat swab specimens. The specific reverse transcription\u2010polymerase chain reaction amplicons labeled with SYBR Orange were separated by microchip capillary electrophoresis and detected by laser induced fluorescence detector within 7 min. The intraday and interday relative standard deviation of migration time for DNA Marker was in the range of 1.36\u20132.94 and 2.78\u20133.96%, respectively. The detection limits were as low as 2.06 \u00d7 10<jats:sup>3<\/jats:sup> copies\/mL for Enterovirus 71 and 5 \u00d7 10<jats:sup>3<\/jats:sup> copies\/mL for Coxsackievirus A16. No cross\u2010reactivity was observed with rotavirus, astrovirus, norovirus, and adenovirus, which showed good specificity of the method. This assay was validated using 100 throat swab specimens that were detected by real\u2010time reverse\u2010transcript polymerase chain reaction in parallel and the two methods produced the same results. This study provided a rapid, sensitive and specific method for the detection of Enterovirus 71 and Coxsackievirus A16, which make a contribution to significant time and cost saving for the identification and treatment of patients.<\/jats:p>","DOI":"10.1002\/jssc.201500165","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T02:35:36Z","timestamp":1431052536000},"page":"2538-2544","source":"Crossref","is-referenced-by-count":6,"title":["Microchip capillary electrophoresis with laser\u2010induced fluorescence combined with one\u2010step duplex reverse\u2010transcription polymerase chain reaction for the rapid detection of Enterovirus 71 and Coxsackievirus A16 in throat swab specimens"],"prefix":"10.1002","volume":"38","author":[{"given":"Ruan","family":"Jia","sequence":"first","affiliation":[{"name":"West China School of Public Health Sichuan University  Chengdu 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