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A hybridoma secreting an anti\u2010glycyrrhizic acid monoclonal antibody was produced by fusing splenocytes from a mouse immunized against a glycyrrhizic acid\u2013bovine serum albumin conjugate with the hypoxanthine\u2013aminopterin\u2013thymidine\u2010sensitive mouse myeloma cell line (Sp2\/0\u2010Ag14). Subsequently, an indirect, competitive enzyme\u2010linked immunosorbent assay for glycyrrhizic acid was developed using the monoclonal antibody. In this assay, we detected an effective measuring range of 78.12\u20132500 ng\/mL. Both intra\u2010assay and inter\u2010assay repeatability and precision were achieved, with relative standard deviations lower than 10%. In addition, glycyrrhizic acid levels in both formulated Chinese medicines and biological samples were determined with high sensitivity and efficiency. We then successfully developed a reliable immunoaffinity chromatography to separate glycyrrhizic acid completely from its parent medicine. These methods will contribute to further research investigations to better understand the interactions of glycyrrhizic acid with other drugs in the complex system of traditional Chinese medicine.<\/jats:p>","DOI":"10.1002\/jssc.201500242","type":"journal-article","created":{"date-parts":[[2015,4,25]],"date-time":"2015-04-25T06:53:28Z","timestamp":1429944808000},"page":"2363-2370","source":"Crossref","is-referenced-by-count":10,"title":["Development of an enzyme\u2010linked immunosorbent assay and immunoaffinity chromatography for glycyrrhizic acid using an anti\u2010glycyrrhizic acid monoclonal antibody"],"prefix":"10.1002","volume":"38","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biopharmaceutics, School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huihua","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Centre of Scientific Experiment Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Chemistry of TCM, School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Basic TCM Clinical Science, School of Basic Medical Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenchao","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Chemistry of TCM, School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Basic TCM Clinical Science, School of Basic Medical Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingguo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Basic TCM Clinical Science, School of Basic Medical Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Basic TCM Clinical Science, School of Basic Medical Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,18]]},"reference":[{"first-page":"80","volume-title":"The Chinese Pharmacopoeia","year":"2010","key":"e_1_2_7_2_1"},{"key":"e_1_2_7_3_1","first-page":"479","volume":"1","author":"Pawar S. 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