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In this method, no microsyringe or fiber is required to support the organic microdrop due to the usage of an organic solvent with a low density and appropriate melting point. Furthermore, the extractant droplet can be collected easily by solidifying it at low temperature. 1\u2010Undecanol and methanol were chosen as extraction and disperser solvents, respectively. Parameters that influence extraction efficiency, i.e. volumes of extracting and dispersing solvents, pH, and salt effect, were optimized by using response surface methodology. Under optimal conditions, enrichment factor for methadone was 134 and 160 in serum and urine samples, respectively. The limit of detection was 3.34\u00a0ng\/mmL in serum and 1.67\u00a0ng\/mL in urine samples. Compared with the traditional dispersive liquid\u2013liquid microextraction, the proposed method obtained lower limit of detection. Moreover, the solidification of floating organic solvent facilitated the phase transfer. And most importantly, it avoided using high\u2010density and toxic solvents of traditional dispersive liquid\u2013liquid microextraction method. The proposed method was successfully applied to the determination of methadone in serum and urine samples of an addicted individual under methadone therapy.<\/jats:p>","DOI":"10.1002\/jssc.201500636","type":"journal-article","created":{"date-parts":[[2015,8,20]],"date-time":"2015-08-20T06:26:19Z","timestamp":1440051979000},"page":"3545-3551","source":"Crossref","is-referenced-by-count":47,"title":["Sensitive determination of methadone in human serum and urine by dispersive liquid\u2013liquid microextraction based on the solidification of a floating organic droplet followed by HPLC\u2013UV"],"prefix":"10.1002","volume":"38","author":[{"given":"Salman","family":"Taheri","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Razi University  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahimeh","family":"Jalali","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Razi University  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazir","family":"Fattahi","sequence":"additional","affiliation":[{"name":"Research Center for Environmental Determinants of Health (RCEDH) Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronak","family":"Jalili","sequence":"additional","affiliation":[{"name":"School of Pharmacy Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gholamreza","family":"Bahrami","sequence":"additional","affiliation":[{"name":"School of Pharmacy Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1001\/jama.1976.03260450029025","volume":"235","author":"Dole V. 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