{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:58:11Z","timestamp":1777424291459,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,7]],"date-time":"2015-12-07T00:00:00Z","timestamp":1449446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81403057, 81473396, 81373369"],"award-info":[{"award-number":["81403057, 81473396, 81373369"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hong Kong, Macau and Taiwan Science & Technology Cooperation Program of China","award":["2014DFH30160"],"award-info":[{"award-number":["2014DFH30160"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Methods based on triple quadrupole tandem mass spectrometry have been widely used and reported as highly selective and sensitive methods for quantifying substances of herbal medicines. However, most of them were limited to targeted components, due to the difficulties to optimize the multiple reaction monitoring transitions without authentic standards. This study proposed a novel strategy for non\u2010targeted optimization of multiple reaction monitoring method based on the diagnostic ion guided family classifications, tandem mass spectrometry database establishment, and transitions and collision energy screening. Applying this strategy, 59 <jats:italic>Fritillaria<\/jats:italic> alkaloids in Fritillariae Ussuriensis Bulbus have been classified, and 51 of these <jats:italic>Fritillaria<\/jats:italic> alkaloids were successfully detected by the optimal multiple reaction monitoring method. For semi\u2010quantification, the easy\u2010to\u2010obtain <jats:italic>Fritillaria<\/jats:italic> alkaloids of each type, such as verticinone for cevanine type and peimisine for jervine type, were used as the reference standards to calibrate the other <jats:italic>Fritillaria<\/jats:italic> alkaloids in the same type. The method was demonstrated a good linearity (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.998) with satisfactory accuracy and precision, and the lower limits of quantification of verticinone and peimisine were estimated to be 0.076 and 0.216 pg, respectively. In addition, the results suggested that the proposed strategy might obtained high quality metabolomics data in discrimination of <jats:italic>Fritillaria unibracteata<\/jats:italic> and <jats:italic>Fritillaria ussuriensis<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201500880","type":"journal-article","created":{"date-parts":[[2015,11,4]],"date-time":"2015-11-04T05:02:48Z","timestamp":1446613368000},"page":"287-295","source":"Crossref","is-referenced-by-count":16,"title":["Global detection and semi\u2010quantification of <i>Fritillaria<\/i> alkaloids in Fritillariae Ussuriensis Bulbus by a non\u2010targeted multiple reaction monitoring approach"],"prefix":"10.1002","volume":"39","author":[{"given":"Li","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong Ping","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) Shenzhen Research Institute of The Hong Kong Polytechnic University  Shenzhen China"},{"name":"Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University  Hung Hom Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si\u2010Bao","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) Shenzhen Research Institute of The Hong Kong Polytechnic University  Shenzhen China"},{"name":"Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University  Hung Hom Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pui\u2010Kin","family":"So","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) Shenzhen Research Institute of The Hong Kong Polytechnic University  Shenzhen China"},{"name":"Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University  Hung Hom Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Qi","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica Jiangsu Provincial Academy of Chinese Medicine  Jiangsu Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) Shenzhen Research Institute of The Hong Kong Polytechnic University  Shenzhen China"},{"name":"Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University  Hung Hom Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui\u2010Zhong","family":"Xin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/448126a"},{"key":"e_1_2_8_3_1","first-page":"789","volume":"86","author":"Patwardhan B.","year":"2004","journal-title":"Curr. 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