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However, the chromatographic peaks were difficult to discriminate clearly in data collection or analysis because of the complexity and the greatly different content of the constituents in TCM formula, which increased the difficulty of identification. In this study, a high\u2010performance liquid chromatography coupled with linear ion trap\u2010Orbitrap mass spectrometry based strategy focused on the comprehensive identification of TCM formula constituents was developed. Identification was carried out from a high dose of medicinal materials to equivalent dose of formula. Meanwhile, combined with mass spectrometry data, chromatographic behaviors, reference standards and previous reports, the identification of constituents in Xiang\u2010Sha\u2010Liu\u2010Jun\u2010Zi\u2010Jia\u2010Jian granules was described. 169 compounds were unambiguously or tentatively characterized, mainly including flavonoids, alkaloids, triterpenic acids, triterpene saponins, lactones, sesquiterpenoids and some other compounds. Among them, 11 compounds were unambiguously confirmed by comparing with reference standards. These results demonstrated that the method was effective and reliable for comprehensive identification of constituents of Xiang\u2010Sha\u2010Liu\u2010Jun\u2010Zi\u2010Jia\u2010Jian granules extracts and reveal the material basis of its therapeutic effects. This strategy might propose a research idea for the characterization of multi\u2010constituents in TCM formula.<\/jats:p>","DOI":"10.1002\/jssc.201501223","type":"journal-article","created":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T03:46:28Z","timestamp":1456976788000},"page":"1619-1627","source":"Crossref","is-referenced-by-count":27,"title":["Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap\u2010Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang\u2010Sha\u2010Liu\u2010Jun\u2010Zi\u2010Jia\u2010Jian granules"],"prefix":"10.1002","volume":"39","author":[{"given":"Fengyun","family":"Wang","sequence":"first","affiliation":[{"name":"Gastroenterology Department, Xiyuan Hospital China Academy of Chinese Medical Sciences  Beijing P.R. 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