{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:27:01Z","timestamp":1783117621328,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,9]],"date-time":"2016-04-09T00:00:00Z","timestamp":1460160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>This work presents a simple, sensitive and generic high\u2010performance liquid chromatography with diode array detection method for the simultaneous determination of seven drugs prescribed for the treatment of erectile dysfunction and premature ejaculation. Investigated drugs include the phosphodiesterase\u20105 inhibitors: sildenafil, tadalafil, and vardenafil, in addition to the selective serotonin reuptake inhibitors: dapoxetine, duloxetine, fluoxetine, and paroxetine. The drugs were separated using a Waters C8 column (4.6 \u00d7 250 mm, 5 \u03bcm) with the mobile phase consisting of phosphate buffer pH 3, acetonitrile and methanol in the ratio 60:33:7. The flow rate was 1.2\u00a0mL\/min, and quantification was based on measuring peak areas at 225 nm. Peaks were perfectly resolved with retention times 3.3, 3.9, 6.4, 7.5, 9.5, 10.7, and 13.4 min for vardenafil, sildenafil, paroxetine, duloxetine, dapoxetine, fluoxetine, and tadalafil, respectively. The developed method was validated with respect to system suitability, linearity, ranges, accuracy, precision, robustness, and limits of detection and quantification. The proposed method showed good linearity in the ranges 5\u2013500, 2\u2013200, 2\u2013200, 3\u2013300, 1.5\u2013150, 2\u2013200, and 2\u2013200 \u03bcg\/mL for sildenafil, tadalafil, vardenafil, dapoxetine, duloxetine fluoxetine, and paroxetine, respectively. The limits of detection were 0.18\u20130.38 \u03bcg\/mL for the analyzed compounds. The applicability of the proposed method to real life situations was assessed through the analysis of commercial tablets, and satisfactory results were obtained.<\/jats:p>","DOI":"10.1002\/jssc.201501339","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T15:14:59Z","timestamp":1457795699000},"page":"1656-1665","source":"Crossref","is-referenced-by-count":34,"title":["High\u2010performance liquid chromatography with diode array detection method for the simultaneous determination of seven selected phosphodiesterase\u20105 inhibitors and serotonin reuptake inhibitors used as male sexual enhancers"],"prefix":"10.1002","volume":"39","author":[{"given":"Mostafa M.","family":"Baker","sequence":"first","affiliation":[{"name":"Methodology Department Pharco Pharmaceuticals Company  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek S.","family":"Belal","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy University of Alexandria  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed S.","family":"Mahrous","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy University of Alexandria  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hytham M.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analysis Department, Faculty of Pharmacy Damanhour University  Damanhour Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda G.","family":"Daabees","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Damanhour University  Damanhour Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.02.020"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287211424172"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767040-00005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/tcrm.2007.3.2.277"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287212453866"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1Q008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000112607"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900841"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.11.024"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400276"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmu010"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4568"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201100849"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e31823bf0fd"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419-012-0152-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN02209B"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.05.050"},{"issue":"4","key":"e_1_2_6_19_1","first-page":"1404","article-title":"Spectrophotometric simultaneous determination of dapoxetine and sildenafil in combined tablet dosage form by absorbance corrected method","volume":"4","author":"Chabukswar A. P.","year":"2012","journal-title":"Der Pharm. Chem."},{"issue":"4","key":"e_1_2_6_20_1","first-page":"393","article-title":"Analytical method development and validation for simultaneous estimation of sildenafil and dapoxetine in bulk & pharmaceutical dosage form by RP\u2010HPLC method","volume":"6","author":"Siddartha B.","year":"2014","journal-title":"Der Pharm. Lett."},{"issue":"10","key":"e_1_2_6_21_1","first-page":"98","article-title":"A novel stability indicating RP\u2010HPLC method for the simultaneous estimation of sildenfil citrate and dapoxetine hydrochloride in bulk and pharmaceutical formulations","volume":"7","author":"Kalyani K.","year":"2015","journal-title":"Der Pharm. Lett."},{"issue":"2","key":"e_1_2_6_22_1","first-page":"654","article-title":"Validated HPLC method for simultaneous quantitation of tadalafil and dapoxetine hydrochloride in bulk drug and formulation","volume":"4","author":"Giri A. D.","year":"2012","journal-title":"Int. J. Pharm. Pharm. Sci."},{"issue":"2","key":"e_1_2_6_23_1","first-page":"72","article-title":"Validated RP\u2010HPLC method for simultaneous estimation of tadalafil and dapoxetine hydrochloride in combined pharmaceutical dosage forms","volume":"4","author":"Rajeshwari M.","year":"2014","journal-title":"Int. J. Pharm. Biol. Sci."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.3724\/SP.J.1123.2015.02063"},{"issue":"5","key":"e_1_2_6_25_1","first-page":"480","article-title":"Simultaneous estimation and validation of vardenafil and dapoxetine hydrochloride in pharmaceutical formulation by thin layer chromatographic densitometric method","volume":"3","author":"Chapla B.","year":"2012","journal-title":"Int. Res. J. Pharm."},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10812-014-9963-0"},{"issue":"10","key":"e_1_2_6_27_1","first-page":"120","article-title":"Spectrophotometric methods for simultaneous determination of vardenafil and dapoxetine hydrochlorides in combined dosage form","volume":"4","author":"Abdel\u2010Moety M. M.","year":"2015","journal-title":"World J. Pharm. Pharm. Sci."},{"key":"e_1_2_6_28_1","volume-title":"Validation of analytical procedures: Text and methodology, Q2(R1)","author":"ICH","year":"2005"},{"key":"e_1_2_6_29_1","unstructured":"http:\/\/www.drugbank.ca\/ (last accessed: February 3 2016)"},{"key":"e_1_2_6_30_1","unstructured":"FDA Reviewer Guidance Validation of chromatographic methods Center for Drug Evaluation and Research (CDER) November1994."},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-120030180"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02746B"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201501339","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201501339","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:45:50Z","timestamp":1760870750000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201501339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,9]]},"references-count":31,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/jssc.201501339"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201501339","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,9]]}}}