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The two\u2010phase solvent system composed of chloroform\/methanol\/0.3 mol\/L hydrochloric acid (4:3:2, v\/v\/v) was selected for separation. From 300 mg of the crude extracts, 56.2 mg of brucine chloromethochloride and 60.2 mg of strychnine chloromethochloride were obtained with the purity of 99.78 and 96.99%, respectively, and the structures were confirmed by mass spectrometry, <jats:sup>1<\/jats:sup>H, <jats:sup>13<\/jats:sup>C, and two\u2010dimensional NMR spectroscopy. The results indicated that the present method is a powerful technology for large\u2010scale isolation of alkaloids from <jats:italic>Semen Strychni<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201600352","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T22:58:27Z","timestamp":1476313107000},"page":"3709-3715","source":"Crossref","is-referenced-by-count":5,"title":["Preparative separation and purification of two highly polar alkaloids derived from <i>Semen Strychni<\/i> extracted with dichloromethane by high\u2010speed countercurrent chromatography"],"prefix":"10.1002","volume":"39","author":[{"given":"Fengsen","family":"Ma","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"The Third Clinical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqiang","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liang","sequence":"additional","affiliation":[{"name":"The Third Clinical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiao","family":"Fang","sequence":"additional","affiliation":[{"name":"The Third Clinical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang Institute for the Control of Medical Device  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,7]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"50","volume-title":"Pharmacopoeia of the People's Republic of China","author":"The State Pharmacopoeia Commission of People's Republic of China","year":"2015"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2012.11.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5246\/jcps.2012.02.025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2014.09.127"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2014.03.028"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3736\/jcim20080620"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.01.012"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.11.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.05.006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2015.05.038"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.08.020"},{"key":"e_1_2_8_13_1","first-page":"113","article-title":"Chemical constituents from dichloromethane extract of Caragana microphylla","volume":"34","author":"Qiu Y. 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